INVIVO METHYLATION AND TURNOVER OF RAT-BRAIN HISTONES
INVIVO METHYLATION AND TURNOVER OF RAT-BRAIN HISTONES
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DOI：
10.1111/j.1471-4159.1974.tb06057.x
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发表时间：
1974-01-01
影响因子：
4.7
通讯作者：
LEE, CT
中科院分区：
文献类型：
--
作者：
DUERRE, JA;LEE, CT
在对新生大鼠给予l-[3 H]赖氨酸和l-[14 C-甲基]蛋氨酸后，研究了来自脑核的不同组蛋白组分的周转。在280天内测定了不同组蛋白亚组分以及其他蛋白质的放射性。全脑组蛋白及其各亚组蛋白的衰变曲线均为3 H和14 C双相型。6 ~ 40日龄组蛋白的半衰期为19天。大脑成熟后，半衰期为132天。富赖氨酸组蛋白（F1）的周转速度最快，半衰期分别为13天和112天。稍微富含赖氨酸的组蛋白（F2 a2，F2 b）的衰变曲线给出了25天至40天龄的半衰期和达到脑成熟后的189天。富含精氨酸的组蛋白（F2 a1，F3）的半衰期为32天至40天的年龄，而成熟后没有观察到营业额。甲基和/或甲硫氨酰残基的周转率没有显着变化的F2和F3组蛋白中的赖氨酰残基的周转率。富含赖氨酸的组蛋白不含大量的甲硫氨酰残基或甲基，对脑组蛋白的氨基酸分析显示，在略微富含赖氨酸的组蛋白中，约3.6%的赖氨酰残基被甲基化，主要是ε-N-二甲基赖氨酸。富含精氨酸的组蛋白中约13%的赖氨酰残基被甲基化，主要是ε-N-单甲基赖氨酸和ε-N-二甲基赖氨酸。
The turnover of the different histone components from brain nuclei was studied after the administration ofl‐[3H]lysine andl‐[14C‐methyl]methionine to newborn rats. The radioactivities of the different histone subfractions as well as other proteins were determined over a 280‐day period. Biphasic type decay curves (3H and14C) were obtained for total brain histones and all the subfractions. From 6 to 40 days of age the half life of total brain histones was 19 days. After reaching brain maturity the half life was 132 days. The lysine rich histone (F1) was found to turnover the fastest of all the histones, having half lives of 13 and 112 days, respectively. The decay curve for the slightly lysine rich histones (F2a2, F2b) gave half lives of 25 days up to 40 days of age and 189 days after reaching brain maturity. The arginine rich histones (F2a1, F3) gave a half life of 32 days up to 40 days of age, while no turnover was observed after maturity. The turnover rates of the methyl groups and/or methionyl residues did not vary significantly from the turnover rates of the lysyl residues in the F2and F3histones. The lysine‐rich histones did not contain significant amounts of methionyl residues or methyl groups.Amino acid analysis of the brain histones revealed that about 3·6 per cent of the lysyl residues in the slightly lysine rich histones were methylated, mainly as ε‐N‐dimethyllysine. About 13 per cent of the lysyl residues in the arginine rich histones were methylated, mainly as ε‐N‐monomethyllysine and ε‐N‐dimethyllysine.