Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway
Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway
复制标题
DOI：
10.1073/pnas.0711034105
复制
发表时间：
2008-03
期刊：
影响因子：
--
通讯作者：
S. Taketa;S. Amano;Y. Tsujino;Tomohiko Sato;D. Saisho;K. Kakeda;M. Nomura;Toshisada Suzuki;
中科院分区：
文献类型：
--
作者：
S. Taketa;S. Amano;Y. Tsujino;Tomohiko Sato;D. Saisho;K. Kakeda;M. Nomura;Toshisada Suzuki;
与其他谷物不同的是，典型的大麦品种在成熟时有附着外壳的颖果，称为盖(壳)麦。然而，一些大麦品种是一种被称为裸(无壳)大麦的自由脱粒变种。遮盖/裸露颖果由染色体臂7HL上的单基因座(NUD)控制。在定位克隆的基础上，我们得出结论，一个乙烯反应因子(ERF)家族转录因子基因控制着遮盖/裸露颖果的表型。这一结论从以下几个方面得到了证实：(I)在所有100个裸品种中定位了含有ERF基因的17kb缺失；(Ii)两个x射线诱导的NUD等位基因在不同的位置发生了DNA损伤，每个等位基因都影响了可能的功能基序；(Iii)基因的表达严格定位于种皮。已有的结果表明，裸大麦的起源是单系起源。Nud基因与拟南芥WIN1/SHN1转录因子基因同源，其推导的功能是控制脂质生物合成途径。用亲脂染料(苏丹黑B)染色，只在盖着的大麦的果皮表皮上检测到一层脂层。我们推断，在盖盖大麦中，颖果表面与外壳内侧覆盖着脂类的接触会产生器官粘连。
In contrast to other cereals, typical barley cultivars have caryopses with adhering hulls at maturity, known as covered (hulled) barley. However, a few barley cultivars are a free-threshing variant called naked (hulless) barley. The covered/naked caryopsis is controlled by a single locus (nud) on chromosome arm 7HL. On the basis of positional cloning, we concluded that an ethylene response factor (ERF) family transcription factor gene controls the covered/naked caryopsis phenotype. This conclusion was validated by (i) fixation of the 17-kb deletion harboring the ERF gene among all 100 naked cultivars studied; (ii) two x-ray-induced nud alleles with a DNA lesion at a different site, each affecting the putative functional motif; and (iii) gene expression strictly localized to the testa. Available results indicate the monophyletic origin of naked barley. The Nud gene has homology to the Arabidopsis WIN1/SHN1 transcription factor gene, whose deduced function is control of a lipid biosynthesis pathway. Staining with a lipophilic dye (Sudan black B) detected a lipid layer on the pericarp epidermis only in covered barley. We infer that, in covered barley, the contact of the caryopsis surface, overlaid with lipids to the inner side of the hull, generates organ adhesion.