Hormone replacement therapy reduces the reactivity of monocytes and platelets in whole blood - A beneficial effect on atherogenesis and thrombus formation?
Hormone replacement therapy reduces the reactivity of monocytes and platelets in whole blood - A beneficial effect on atherogenesis and thrombus formation?
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DOI：
10.1016/0002-9378(95)90433-6
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发表时间：
1995-12-01
影响因子：
9.8
通讯作者：
Osterud, B
中科院分区：
文献类型：
--
作者：
Aune, B;Oian, P;Osterud, B
目的：探讨激素替代治疗对全血单核细胞和血小板反应性的影响。方法：采用组织因子活性、肿瘤坏死因子-α和血栓素B-2测定。研究设计：32例妇女随机分为经皮和口服联合激素替代治疗，分别于治疗前、治疗3个月和12个月采血。检测未经刺激的全血和弱脂多糖刺激后单核细胞中组织因子的活性。结果：经过12个月的激素替代治疗后，未刺激和脂多糖刺激的单核细胞组织因子活性均显著降低(p<；0.001)，肿瘤坏死因子-α和血栓素B-2的形成显著减少(p<；0.03和p<；0.02)。透皮组和口服组之间的这些参数没有差异。治疗3个月后未见变化。结论：12个月的激素替代治疗降低了单核细胞和血小板的细胞活性；这些变化可能是降低心血管疾病风险的一些有益效果。
OBJECTIVE: Our purpose was to investigate the effects of hormone replacement therapy on the reactivity of monocytes and platelets in whole blood, measured by tissue factor activity, tumor necrosis factor-alpha, and thromboxane B-2.STUDY DESIGN: Thirty-two women were randomized into either transdermal or oral combined hormone replacement therapy and underwent blood sampling before and after 3 and 12 months of treatment. The tissue factor activity in monocytes was measured both in unstimulated whole blood and after a weak lipopolysaccharide stimulation. Tumor necrosis factor-alpha and thromboxane B-2 formation in plasma were measured after a weak lipopolysaccharide stimulation of whole blood.RESULTS: After 12 months of hormone replacement therapy there were significant reductions of tissue factor activity in both unstimulated and lipopolysaccharide-stimulated monocytes (p < 0.001) and significant reductions in the formation of tumor necrosis factor-alpha (p < 0.03) and thromboxane B-2 (p < 0.02). There were no differences in these parameters between the transdermal and the oral groups. No changes were observed after 3 months of therapy.CONCLUSION: Twelve months of hormone replacement therapy reduces cellular activation of blood monocytes and platelets; these changes may account for some of the beneficial effects in reducing the risk of cardiovascular disease.