Ultrasound indentation of normal and spontaneously degenerated bovine articular cartilage
Ultrasound indentation of normal and spontaneously degenerated bovine articular cartilage
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DOI：
10.1016/s1063-4584(03)00154-7
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发表时间：
2003-09-01
影响因子：
7
通讯作者：
Töyräs, J
中科院分区：
文献类型：
--
作者：
Saarakkala, S;Laasanen, MS;Töyräs, J
目的：研制一种用于诊断软骨退变的手持式超声压痕仪。该仪器已被证明是能够量化的机械和声学性能的酶降解和正常牛关节软骨在体外和原位。本研究的目的是研究仪器区分正常和自发变性（例如，设计：从牛外侧髌骨制备30个关节软骨样品：19个具有不同退行性阶段的髌骨和11个具有视觉正常外观的髌骨。用手持式仪器测量软骨厚度、刚度（动态模量）和来自软骨表面的超声反射。结果：超声压痕仪测量结果的重现性良好。厚度、动模量和反射系数的标准化变异系数均小于或等于6.1%。超声压痕仪测得的动态模量与参考动态模量呈高度线性相关（r = 0.993，n = 30，P < 0.05）。从软骨表面测得的超声反射系数与软骨成分、组织学或力学性质呈高度线性相关（r（2）> 0.64，n = 30，P < 0.05）。该仪器是上级相比，视觉评价在检测组织degeneration.Conclusion：这项研究表明，超声压痕技术和仪器可以显着提高软骨退变的早期诊断。结果表明，视觉评价是不敏感的估计关节软骨的结构和力学性能在退变的初始阶段。(C)2003年由Elsevier Ltd代表国际骨关节炎研究学会出版。
Objective: We have previously developed a handheld ultrasound indentation instrument for the diagnosis of cartilage degeneration. The instrument has been demonstrated to be capable of quantifying mechanical and acoustic properties of enzymatically degraded and normal bovine articular cartilage in vitro and in situ. The aim of this study was to investigate the sensitivity of the instrument to distinguish between normal and spontaneously degenerated (e.g., in osteoarthrosis) articular cartilage in vitro.Design: Thirty articular cartilage samples were prepared from the bovine lateral patellae: 19 patellae with different degenerative stages and 11 patellae with visually normal appearance. Cartilage thickness, stiffness (dynamic modulus) and ultrasound reflection from the cartilage surface were measured with the handheld instrument. Subsequently, biomechanical, histological and biochemical reference measurements were conducted.Results: Reproducibility of the measurements with the ultrasound indentation instrument was good. Standardized coefficient of variation was less than or equal to 6.1% for thickness, dynamic modulus and reflection coefficient. Linear correlation between the dynamic modulus, measured with the ultrasound indentation instrument, and the reference dynamic modulus was high (r = 0.993, n = 30, P < 0.05). Ultrasound reflection coefficient, as determined from the cartilage surface, showed high linear correlations (typically r(2) > 0.64, n = 30, P < 0.05) with the cartilage composition and histological or mechanical properties. The instrument was superior compared to visual evaluation in detecting tissue degeneration.Conclusion: This study indicates that the ultrasound indentation technique and instrument may significantly improve the early diagnosis of cartilage degeneration. The results revealed that visual evaluation is insensitive for estimating the structural and mechanical properties of articular cartilage at the initial stages of degeneration. (C) 2003 Published by Elsevier Ltd on behalf of OsteoArthritis Research Society International.