Expression of Salivary S100A7 Levels in Stage I Oral Submucous Fibrosis: A Clinical and Laboratory Study.
Expression of Salivary S100A7 Levels in Stage I Oral Submucous Fibrosis: A Clinical and Laboratory Study.
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DOI：
10.31557/apjcp.2020.21.4.1115
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发表时间：
2020-04-01
期刊：
影响因子：
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通讯作者：
Vohra, Fahim
中科院分区：
文献类型：
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作者：
Raffat, Muhammad Arsalan;Hadi, Naila Irum;Vohra, Fahim
背景：口腔黏膜下纤维化（OSF）是一种以上皮旁纤维化为特征的慢性衰弱性疾病。与高危癌前病变相关的主要病因是槟榔的使用。S100A7是脊椎动物中发现的最大的钙结合蛋白的成员，与许多细胞内和细胞外功能的调节有关。本研究的目的是研究S100A7蛋白在I期OSF患者和健康对照者唾液样本中的表达。方法：本研究纳入63名参与者，其中30名患有OSF I期，33名健康对照。采用非概率配额抽样技术招募研究参与者。采用结构化基线问卷收集人口统计数据。采用被动钻取法在无菌容器中采集唾液样本。采用酶联免疫吸附法测定唾液中S100A7的水平。对数据的正态性进行夏皮罗威尔克检验。采用学生t检验，评价两组间S100A7蛋白的表达。结果：ⅰ期OSF组唾液S100A7平均值为0.334 ng/ml，健康对照组为0.172 ng/ml。学生t检验报告差异有统计学意义，第一阶段OSF组S100A7水平高于健康对照组（p < 0.001）。在个体组分析中，唾液S100A7与槟榔食用时间（r = -0.45, p = 0.009）和咀嚼槟榔（r = -0.20, p = 0.03）呈显著负相关，唾液S100A7与开口度（r = 0.03, p = 0.04）呈显著正相关。结论：与健康个体相比，OSF I期组S100A7蛋白水平较高。我们的研究结果表明，S100A7可作为识别OSF发展风险患者的替代评估。
BACKGROUND: Oral submucous fibrosis (OSF) is a chronic debilitating condition characterized by juxta-epithelial fibrosis. The main etiological agent associated with the high-risk precancerous condition is areca nut use. S100A7 is a member of the largest calcium-binding proteins exclusively found in vertebrates and are associated with the regulation of numerous intracellular and extracellular functions. The aim of this study was to investigate the expression of protein S100A7 in salivary samples of individuals with stage I OSF and healthy controls.METHODS: This study included 63 participants, 30 of whom had OSF stage I and 33 healthy controls. Nonprobability quota sampling technique was utilized for recruitment of the study participants. A structured baseline questionnaire was used to collect demographic data. Saliva samples were collected by passive droll technique in a sterile container. Salivary levels of S100A7 were quantified by enzyme-linked immunosorbent assay. For the normality of the data Shapiro Wilk test was performed. Student t-test was commuted to evaluate the expression of S100A7 protein expression between both the study groups.RESULTS: The mean salivary S100A7 value for stage I OSF group was 0.334 ng/ml, compared to 0.172 ng/ml for healthy controls. Student t-test reported a statistically significant difference, indicating higher levels of S100A7 in stage I OSF group than in healthy controls (p < 0.001). In the individual group analysis, a significant negative correlation was found between salivary S100A7 and duration of areca nut use (r = -0.45, p = 0.009) and gutka chewing (r = -0.20, p = 0.03), while a significant positive correlation was found between salivary S100A7 and mouth opening (r = 0.03, p = 0.04).CONCLUSIONS: Higher levels of S100A7 protein level was seen in stage I OSF group in comparison to the healthy individuals. Results of our study suggest that S100A7 could be used as a surrogate assessment to identify patients at risk of OSF development..