INFLUENCE OF SOME FACTORS ON LIPOIDOSIS AND CELL PROLIFERATION IN AORTA TISSUE CULTURES OF ADULT RABBITS
INFLUENCE OF SOME FACTORS ON LIPOIDOSIS AND CELL PROLIFERATION IN AORTA TISSUE CULTURES OF ADULT RABBITS
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DOI：
10.1016/s0368-1319(65)80006-0
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发表时间：
1965-01-01
期刊：
影响因子：
--
通讯作者：
BLOCK, YE
中科院分区：
文献类型：
--
作者：
MYASNIKO.AL;BLOCK, YE
本文介绍了在同种营养液中培养成年家兔完整的和动脉粥样硬化的升动脉组织的方法。用正常血清培养的完整的大肠杆菌的培养物中的脂肪积累的动力学可以被描述为细胞中脂质含量的节律性变化。没有观察到的节奏动力学与贫血血清，其中的程度的脂质沉积和细胞变性的文化是更大的，和脂质沉积的最后阶段（脂肪变性和死亡的细胞）发生早于在正常血清的文化。同时，在培养的初始阶段，高脂血清比正常血清更能刺激培养物中的细胞迁移和细胞增殖。营养培养基中抗坏血酸浓度增加0.5 mg/100 ml可降低高脂血清培养物中的脂质沉积，并增加正常血清培养物中的脂质沉积。在正常血清中培养的动脉粥样硬化动脉瘤细胞的脂质沉积比同龄的完整动脉瘤细胞的脂质沉积更不明显。
Methods of cultivating tissues of intact and atherosclerotic ascending aortae of adult rabbits in homologous nutrient media are described. The dynamics of fat accumulation in the cultures of intact aortae cultivated with the normal serum can be described as a rhythmic change of lipid content in the cells. The rhythmic dynamics were not observed in cultures with hyperlipemic serum, where the degree of lipoidosis and cell degeneration was greater, and the final stages of lipoidosis (fat degeneration and death of the cells) occurred earlier than in cultures with normal serum. At the same time the hyper- lipemic serum was a stronger stimulant of cell migration and cell proliferation in cultures than was normal serum at the initial stages of cultivation. A 0.5 mg/100 ml increase in ascorbic acid concentration in the nutrient medium decreased lipoidosis in cultures with the hyper-lipemic serum and increased it in cultures with the normal serum. The lipoidosis of cells in cultures of atherosclerotic aortae grown with the normal serum was less pronounced than in cultures of intact aortae of the same age.