An antibody profile of systemic lupus erythematosus detected by antigen microarray
An antibody profile of systemic lupus erythematosus detected by antigen microarray
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DOI：
10.1111/j.1365-2567.2010.03245.x
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发表时间：
2010-07-01
期刊：
影响因子：
6.4
通讯作者：
Cohen, Irun R.
中科院分区：
文献类型：
--
作者：
Fattal, Ittai;Shental, Noam;Cohen, Irun R.
系统性红斑狼疮（SLE）患者产生针对许多不同自身抗原的抗体。在这里，我们使用含有数百种抗原的抗原微阵列研究SLE血清中的抗体，其中大多数是自身抗原。目的是检测SLE患者在不同临床状态下的抗体反应性特征，包括急性狼疮肾炎的SLE患者、肾脏缓解的SLE患者和从未有肾脏受累的SLE患者。该分析产生了两个新的发现：(i) SLE抗体谱持续存在，独立于疾病活动，尽管长期临床缓解；（ii） SLE抗体谱包括四种特异性免疫球蛋白G （IgG）对双链DNA （dsDNA）、单链DNA （ssDNA）、eb病毒（EBV）和透明质酸的反应性增加；该特征还包括IgM对髓过氧化物酶（MPO）、CD99、III型胶原、胰岛素样生长因子结合蛋白1 （IGFBP1）和心磷脂的特异性反应性降低。两种反应性共同显示SLE的高敏感性（> 93%）和高特异性（> 88%）。有SLE抗体谱的健康对照受试者后来被发现发展为临床SLE。本研究未发现SLE患者不同亚组间的抗体反应性差异有统计学意义。因此，SLE的特点是不论临床状态如何都具有持久的抗体谱。SLE与IgM天然自身抗体降低的关联表明，这些自身抗体可能增强对SLE的抵抗力。
P>Patients with systemic lupus erythematosus (SLE) produce antibodies to many different self-antigens. Here, we investigated antibodies in SLE sera using an antigen microarray containing many hundreds of antigens, mostly self-antigens. The aim was to detect sets of antibody reactivities characteristic of SLE patients in each of various clinical states - SLE patients with acute lupus nephritis, SLE patients in renal remission, and SLE patients who had never had renal involvement. The analysis produced two novel findings: (i) an SLE antibody profile persists independently of disease activity and despite long-term clinical remission, and (ii) this SLE antibody profile includes increases in four specific immunoglobulin G (IgG) reactivities to double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), Epstein-Barr virus (EBV) and hyaluronic acid; the profile also includes decreases in specific IgM reactivities to myeloperoxidase (MPO), CD99, collagen III, insulin-like growth factor binding protein 1 (IGFBP1) and cardiolipin. The reactivities together showed high sensitivity (> 93%) and high specificity for SLE (> 88%). A healthy control subject who had the SLE antibody profile was later found to develop clinical SLE. The present study did not detect antibody reactivities that differentiated among the various subgroups of SLE subjects with statistical significance. Thus, SLE is characterized by an enduring antibody profile irrespective of clinical state. The association of SLE with decreased IgM natural autoantibodies suggests that these autoantibodies might enhance resistance to SLE.