Investigating the mechanism of thylakoid direct electron transfer for photocurrent generation
Investigating the mechanism of thylakoid direct electron transfer for photocurrent generation
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DOI：
10.1016/j.electacta.2013.06.081
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发表时间：
2014-04-20
影响因子：
6.6
通讯作者：
Minteer, Shelley D.
中科院分区：
文献类型：
--
作者：
Rasmussen, Michelle;Minteer, Shelley D.
研究了包含菠菜类囊体的生物阳极，以确定电子直接转移到碳电极的机制。这些电极产生 0.43 +/- 0.02 mu A/cm(2) 的光电流。当类囊体的各个成分被去除、抑制或激活时，测量光电流的变化，以确定哪些成分对光电流有贡献。结果表明，光系统 I 和 II、质体醌、细胞色素 b(6)f 和质体蓝素参与直接电子传递机制，并且光合作用中的两种电子传递途径（循环和非循环）都有助于光电流。使用细胞器作为生物催化剂的好处之一是可以在电极表面形成多个电子转移途径。报道的类囊体电极显示了光合作用前五个电子转移步骤中电子的贡献，只有最后两个步骤不参与。 (C) 2013 Elsevier Ltd. 保留所有权利。
Bioanodes incorporating thylakoids from spinach were studied to determine the mechanism of direct electron transfer to carbon electrodes. These electrodes generated a photocurrent of 0.43 +/- 0.02 mu A/cm(2). The change in this photocurrent was measured when individual components of thylakoids were removed, inhibited, or activated in order to determine which components contributed to the photocurrent. The results indicate that photosystems I and II, plastoquinone, cytochrome b(6)f, and plastocyanin are involved in the direct electron transfer mechanism and both electron transport pathways in photosynthesis (cyclic and noncyclic) contribute to the photocurrent. One of the benefits to using an organelle as a biocatalyst is the possibility for multiple electron transfer pathways at the electrode surface. The reported thylakoid electrodes show a contribution of electrons from the first five electron transfer steps in photosynthesis with only the last two steps not participating. (C) 2013 Elsevier Ltd. All rights reserved.