Characterization of the Arabidopsis thaliana exocyst complex gene families by phylogenetic, expression profiling, and subcellular localization studies
Characterization of the Arabidopsis thaliana exocyst complex gene families by phylogenetic, expression profiling, and subcellular localization studies
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DOI：
10.1111/j.1469-8137.2009.03070.x
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发表时间：
2010-01-01
期刊：
影响因子：
9.4
通讯作者：
Goring, Daphne R.
中科院分区：
文献类型：
--
作者：
Chong, Yolanda T.;Gidda, Satinder K.;Goring, Daphne R.
外囊是由8种蛋白质（Sec 3 p、Sec 5 p、Sec 6p、Sec 8 p、Sec 10 p、Sec 15 p、Exo 70 p和Exo 84 p）组成的复合物，在调节或极化分泌过程中参与将囊泡束缚在质膜上。本论文对植物外囊复合体的系统发育、表达和亚细胞定位进行了研究，并在拟南芥、水稻、大果杨和小立碗藓的全基因组中对预测的外囊亚基的进化关系进行了分析。此外，详细的表达谱的A.利用拟南芥基因芯片数据库，研究了几种植物外囊亚基基因在同一个祖先中的基因扩增和随后在独立的植物谱系中的重复事件。表达谱分析显示，A.拟南芥Exo 70基因家族表现出动态的表达模式，而其余的外囊亚基基因表现出更多的静态模式。亚细胞定位模式A。thaliana外囊亚基的范围从胞质到内体区室（在早期内体和trans-Golgi网络中富集）。有趣的是，两个内体定位的AtExo 70蛋白也招募其他外囊亚基到这些compartment.Overall亚细胞定位模式，也被发现在酵母和动物细胞中，这一点，再加上进化关系，表明外囊可能在植物中执行类似的保守功能。
P>The exocyst is a complex of eight proteins (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p and Exo84p) involved in tethering vesicles to the plasma membrane during regulated or polarized secretion. Here, the plant exocyst complex was explored in phylogenetic, expression, and subcellular localization studies.Evolutionary relationships of predicted exocyst subunits were examined in the complete genomes of Arabidopsis thaliana, Oryza sativa, Populus trichocarpa and Physcomitrella patens. Furthermore, detailed expression profiling of the A. thaliana microarray databases was performed and subcellular localization patterns were studied.Several plant exocyst subunit genes appear to have undergone gene expansion in a common ancestor and subsequent duplication events in independent plant lineages. Expression profiling revealed that the A. thaliana Exo70 gene family exhibits dynamic expression patterns, while the remaining exocyst subunit genes displayed more static profiles. Subcellular localization patterns for A. thaliana exocyst subunits ranged from cytosolic to endosomal compartments (with enrichment in the early endosomes and the trans-Golgi network). Interestingly, two endosomal-localized AtExo70 proteins also recruited other exocyst subunits to these compartments.Overall subcellular localization patterns were observed that were also found in yeast and animal cells, and this, coupled with the evolutionary relationships, suggests that the exocyst may perform similar conserved functions in plants.