Acacia honey accelerates in vitro corneal ulcer wound healing model
Acacia honey accelerates in vitro corneal ulcer wound healing model
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DOI：
10.1186/s12906-016-1248-0
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发表时间：
2016-07-29
影响因子：
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通讯作者：
Ngah, Wan Zurinah Wan
中科院分区：
文献类型：
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作者：
Abd Ghafar, Norzana;Ker-Woon, Choy;Ngah, Wan Zurinah Wan
背景资料：本研究旨在评价阿拉伯胶蜂蜜（AH）对培养的兔角膜成纤维细胞迁移、分化和愈合特性的影响。方法：分离新西兰白色兔角膜基质成纤维细胞（n = 6），培养至第1代。使用4 mm角膜环钻在融合培养物上产生体外角膜溃疡，并用基础培养基（FD）、含血清培养基（FDS）（含和不含0.025% AH）处理。在伤口形成后第0、3和6天记录伤口面积。分别采用qRT-PCR和免疫细胞化学方法检测与伤口愈合和分化相关的基因和蛋白，如醛脱氢酶（ALDH）、波形蛋白、α-平滑肌肌动蛋白（α-SMA）、I型胶原、Lumican和基质金属蛋白酶12（MMP 12）。在富含AH的FDS培养基中培养的细胞增加波形蛋白、I型胶原和Lumican基因的表达，并降低ALDH、α-SMA和MMP 12基因的表达。结论：AH可促进角膜成纤维细胞的迁移和分化，同时增加与角膜基质创伤愈合相关的基因和蛋白表达。
Background: The study aimed to evaluate the effects of Acacia honey (AH) on the migration, differentiation and healing properties of the cultured rabbit corneal fibroblasts.Methods: Stromal derived corneal fibroblasts from New Zealand White rabbit (n = 6) were isolated and cultured until passage 1. In vitro corneal ulcer was created using a 4 mm corneal trephine onto confluent cultures and treated with basal medium (FD), medium containing serum (FDS), with and without 0.025 % AH. Wound areas were recorded at day 0, 3 and 6 post wound creation. Genes and proteins associated with wound healing and differentiation such as aldehyde dehydrogenase (ALDH), vimentin, alpha-smooth muscle actin (alpha-SMA), collagen type I, lumican and matrix metalloproteinase 12 (MMP12) were evaluated using qRT-PCR and immunocytochemistry respectively.Results: Cells cultured with AH-enriched FDS media achieved complete wound closure at day 6 post wound creation. The cells cultured in AH-enriched FDS media increased the expression of vimentin, collagen type I and lumican genes and decreased the ALDH, alpha-SMA and MMP12 gene expressions. Protein expression of ALDH, vimentin and a-SMA were in accordance with the gene expression analyses.Conclusion: These results demonstrated AH accelerate corneal fibroblasts migration and differentiation of the in vitro corneal ulcer model while increasing the genes and proteins associated with stromal wound healing.