A PCR survey for posterior Hox genes in amphibians
A PCR survey for posterior Hox genes in amphibians
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DOI：
10.1016/j.ympev.2005.08.012
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发表时间：
2006-02-01
影响因子：
4.1
通讯作者：
Leyns, L
中科院分区：
文献类型：
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作者：
Mannaert, A;Roelants, K;Leyns, L
Hox基因编码的转录因子在后生动物的身体规划中起着关键作用，因此在解释进化多样性模式方面至关重要。两栖动物是一个古老的四足动物类群，其肢体类型多样，对于了解陆生脊椎动物肢体的起源和多样性具有重要意义。我们进行了PCR调查，在两个物种的每种两栖动物，以确定Hox-9至Hox-13，已知的功能在肢体发育。鉴定了15个不同的后部Hox基因和1个逆转录假基因，前者证实了每个两栖动物目中存在4个Hox簇。基于哺乳动物、鱼类和腔棘鱼的后Hox补体，一些预期出现在所有四足动物中的基因没有从我们的调查中恢复，并且可能已经丢失。Hoxd-12在青蛙和其他两栖动物中可能不存在。考虑到它在伪足发育中的功能，该基因的缺失可能与青蛙和蝾螈中第五指的缺失有关。(c)2005年爱思唯尔公司All rights reserved.
Hox genes encode transcription factors that play a key role in specifying the body plan in metazoans and are therefore essential in explaining patterns of evolutionary diversity. As an ancient tetrapod group with diverse limb types, amphibians are important for understanding the origin and diversification of limbs in land vertebrates. We conducted a PCR survey in two species of each amphibian order to identify Hox-9 to Hox-13, known to function in limb development. Fifteen distinct posterior Hox genes and one retro-pseudogene were identified, and the former confirm the existence of four Hox clusters in each amphibian order. Some genes expected to occur in all tetrapods, based on the posterior Hox complement of mammals, fishes and coelacanth, were not recovered from our survey, and may have been lost. Hoxd-12 is absent in frogs and possibly other amphibians. Considering its function in autopodial development, the loss of this gene may be related to the absence of the fifth finger in frogs and salamanders. (c) 2005 Elsevier Inc. All rights reserved.