Major related sets of antibiotic-resistant pneumococci in the United States as determined by pulsed-field gel electrophoresis and pbp1a-pbp2b-pbp2x-dhf restriction profiles
Major related sets of antibiotic-resistant pneumococci in the United States as determined by pulsed-field gel electrophoresis and pbp1a-pbp2b-pbp2x-dhf restriction profiles
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DOI：
10.1086/315194
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发表时间：
2000-01-01
影响因子：
6.4
通讯作者：
Beall, B
中科院分区：
文献类型：
--
作者：
Gherardi, G;Whitney, CG;Beall, B
为了评估美国引起严重疾病的肺炎球菌的遗传多样性，对来自7个人口中心的241株最近的无菌场所分离株进行了3种青霉素结合蛋白（PBP）基因扩增子和DHF扩增子的限制性图谱检查，该分析提供了对流行病学研究有用的标记，并且通常预测对β-内酰胺类抗生素和甲氧苄啶-磺胺甲恶唑的耐药性。8种脉冲场凝胶电泳（PFGE）类型，每种代表3-40个分离株，占144个β-内酰胺耐药肺炎球菌中的134个（青霉素、头孢噻肟或两者的MIC大于或等于1 μ g/mL）。这些PFGE类型中有五种包含与先前表征的肺炎球菌克隆亚型高度相关的亚型。在这些PFGE类型的4，主要的复合PBP基因-dhf配置文件是高度相关的复合配置文件从以前的特点相关的克隆。在144株β-内酰胺类耐药肺炎球菌中发现8种荚膜血清型。具有相同PBP基因-dhf谱和相关PFGE型的分离株之间的不同荚膜类型表明了几种荚膜血清型转换的情况。
To assess the genetic diversity of pneumococci causing serious disease within the United States, restriction profiles of 3 penicillin-binding protein (PBP)-gene amplicons and the dhf amplicon were examined in 241 recent sterile-site isolates from 7 population centers, This analysis provided markers useful for epidemiologic studies and was generally predictive of resistances to beta-lactam antibiotics and trimethoprim-sulfamethoxazole. Eight pulsed-field gel electrophoresis (PFGE) types, each representing 3-40 isolates, accounted for 134 of the 144 beta-lactam-resistant pneumococci (MICs greater than or equal to 1 mu g/mL for penicillin, cefotaxime, or both). Five of these PFGE types contained subtypes highly related to subtypes of previously characterized pneumococcal clones. Within 4 of these PFGE types, the major composite PBP gene-dhf profile was highly related to the composite profile from the previously characterized related clone. Eight capsular serotypes were found among the 144 beta-lactam-resistant pneumococci, Divergent capsular types among isolates with identical PBP gene-dhf profiles and related PFGE types indicated several instances of capsular serotype switching.