Quantification of ethanol in plasma by electrochemical detection with an unmodified screen printed carbon electrode.
Quantification of ethanol in plasma by electrochemical detection with an unmodified screen printed carbon electrode.
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使用未经修饰的丝网印刷碳电极通过电化学检测对血浆中的乙醇进行定量
DOI：
10.1038/srep23569
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发表时间：
2016-03-23
影响因子：
4.6
通讯作者：
Ding M
中科院分区：
文献类型：
--
作者：
Tian G;Zhang XQ;Zhu MS;Zhang Z;Shi ZH;Ding M
简单、快速、准确地检测血液中乙醇浓度对潜在急性乙醇中毒患者的诊断和治疗至关重要。建立了一种无需样品制备的一次性未改性丝网印刷碳电极（SPCE）定量人血浆中乙醇的电化学检测方法。乙醇脱氢酶（ADH）与辅助因子烟酰胺腺嘌呤二核苷酸（NAD+）的反应产物间接检测乙醇。方法验证结果表明，定量精密度好，日内、日间相对标准偏差分别≤9.4%和8.0%。血浆乙醇浓度在0.10 ~ 3.20 mg/mL范围内呈线性关系，检出限为40.0 μg/mL （S/N >.3）。在20份人血浆样品中，该方法与顶空气相色谱法具有良好的相关性（相关系数为0.9311）。该方法可用于急性乙醇中毒或酒精相关性死亡的诊断。
Simple, rapid and accurate detection of ethanol concentration in blood is very crucial in the diagnosis and management of potential acute ethanol intoxication patients. A novel electrochemical detection method was developed for the quantification of ethanol in human plasma with disposable unmodified screen-printed carbon electrode (SPCE) without sample preparation procedure. Ethanol was detected indirectly by the reaction product of ethanol dehydrogenase (ADH) and cofactor nicotinamide adenine dinucleotide (NAD+). Method validation indicated good quantitation precisions with intra-day and inter-day relative standard deviations of ≤9.4% and 8.0%, respectively. Ethanol concentration in plasma is linear ranging from 0.10 to 3.20 mg/mL, and the detection limit is 40.0 μg/mL (S/N > 3). The method shows satisfactory correlation with the reference method of headspace gas chromatography in twenty human plasma samples (correlation coefficient 0.9311). The proposed method could be applied to diagnose acute ethanol toxicity or ethanol-related death.