rRNA intergenic spacer regions for phylogenetic analysis of Rickettsia species
rRNA intergenic spacer regions for phylogenetic analysis of Rickettsia species
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DOI：
10.1111/j.1749-6632.2003.tb07451.x
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发表时间：
2003-01-01
期刊：
影响因子：
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通讯作者：
Tenreiro, R
中科院分区：
文献类型：
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作者：
Vitorino, L;Zé-Zé, L;Tenreiro, R
立克次体属的物种是导致几种人类疾病的原因，即流行性斑疹伤寒、落基山斑疹热和由节肢动物媒介传播的蜱传斑疹伤寒。对12株立克次体rrl-rrf基因间隔区（rrl-rrf ITS）进行了序列测定。伤寒沙门氏菌6种，R. aeschlimannii、Bar 29、黑脉弯孢霉R. helvetica，R.霍内河和R. slovaca以及5个葡萄牙分离株。从rrl-rrf间隔区序列推断的系统发育树，使用最大简约和距离方法提供了很大程度上全等的树拓扑结构，支持显着的自举值，使五个不同的立克次体集群的识别。
Species of the genus Rickettsia are responsible for several human diseases, namely epidemic typhus, Rocky Mountain spotted fever, and tickborne typhus transmitted by arthropod vectors. The rrl-rrf intergenic spacer region (rrl-rrf ITS) was sequenced for 12 Rickettsia strains, including R. typhi, 6 untested species, R. aeschlimannii, Bar29, R. helvetica, R. honei, R. massilae, and R. slovaca as well as 5 Portuguese isolates. Phylogenetic trees inferred from rrl-rrf spacer sequences using maximum-parsimony and distance methods provided largely congruent tree topologies, supported by significant bootstrap values, enabling the identification of five distinct rickettsiae clusters.