Epitope analysis of Japanese cedar pollen allergen Cry j1 with the human monoclonal antibody 4701-1.
Epitope analysis of Japanese cedar pollen allergen Cry j1 with the human monoclonal antibody 4701-1.
复制标题
使用人单克隆抗体 4701-1 对日本柳杉花粉过敏原 Cry j1 进行表位分析。
作者：
Y. Naganawa;M. Takeda;M. Shimmoto;H. Shinmoto
我们获得了一个稳定的人-小鼠杂交瘤克隆4701-1，能分泌IgM类人抗杉木花粉过敏原Cry j1的单克隆抗体。用合成的五肽进行针肽酶联免疫吸附试验（ELISA），发现LYTVT NSDDD PVNPA氨基酸序列的肽段阳性。对十肽至四肽的详细分析表明，TVTN的一个氨基酸序列是抗体结合的必需序列。当N （Asn）被TVTN表位的A （Ala）取代时，得到的肽不具有抗体结合能力。我们认为，TVTN序列可能在Cry j1特异性B细胞作为抗原提呈细胞对Cry j1过敏原的早期识别中起重要作用。
We obtained a stable human-mouse hybridoma clone 4701-1 secreting IgM class human monoclonal antibody to Japanese cedar pollen allergen Cry j1. A pin-peptide enzyme-linked immunosorbent assay (ELISA) with synthesized pentadeca peptides showed a peptide with an amino acid sequence of LYTVT NSDDD PVNPA was found to be positive. Detailed analysis with deca to tetra peptides indicated that an amino acid sequence of TVTN was an essential sequence for antibody binding. When N (Asn) was substituted with A (Ala) of the TVTN epitope, the resulting peptide did not have antibody binding ability. We concluded that the TVTN sequence might have a very important role in early recognition of Cry j1 allergen by Cry j1-specific B cells, which act as antigen presenting cells.
Epstein Barr 病毒克隆转化的人 B 淋巴细胞表达免疫球蛋白。
DOI：
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发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Brown,NA;Miller,G
通讯作者：
Miller,G