Effects of Exogenous Abscisic Acid on Cell Membrane and Endogenous Hormone Contents in Leaves of Sugarcane Seedlings under Cold Stress
Effects of Exogenous Abscisic Acid on Cell Membrane and Endogenous Hormone Contents in Leaves of Sugarcane Seedlings under Cold Stress
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DOI：
10.1007/s12355-014-0343-0
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发表时间：
2015-03-01
期刊：
影响因子：
1.9
通讯作者：
Wu, Jian-Ming
中科院分区：
文献类型：
--
作者：
Huang, Xing;Chen, Ming-Hui;Wu, Jian-Ming
甘蔗是我国乃至世界最重要的糖料作物，原产于热带、亚热带地区，是喜温作物。近年来，世界范围内极端天气频繁发生，造成严重的低温或霜冻灾害，给甘蔗生产造成巨大损失。脱落酸（阿坝）调节植物许多重要的生理生化过程，诱导植物对包括冷害和霜冻在内的各种逆境的耐受性。以耐冷甘蔗品种桂糖28和感冷甘蔗品种粤糖6号为材料，研究了低温诱导阿坝生物合成与内源激素平衡的关系。在冷处理前12小时用阿坝喷洒植物，与对照组相反，对照组中没有添加额外的物质。当对照组中的植物暴露于冷胁迫时，植物细胞膜受到损伤，并且GA（3）（赤霉酸3）降低，而相对电导率、MDA（3）、阿坝/IAA比值（吲哚乙酸），和阿坝/ZR的比例在低温胁迫下，玉米素（Zeatin Riboside）含量均增加，脯氨酸、脱落酸（阿坝）和赤霉素（GA）含量的变化存在基因型差异，低温胁迫下，抗寒品种桂糖28与抗寒品种粤糖6之间存在阿坝/GA比值。抗寒性强的品种脯氨酸、阿坝含量高，阿坝/GA比值高，GA含量低，这是造成两个甘蔗品种抗寒性不同的重要生理基础。阿坝处理能有效地减轻细胞膜损伤，降低MDA和GA（3）含量，提高脯氨酸、阿坝含量和阿坝/GA（3）比值。阿坝处理组甘蔗叶片中MDA和GA（3）含量降低，而脯氨酸、阿坝含量和阿坝/GA（3）比值升高，是提高甘蔗耐冷性的重要因素。
Sugarcane is the most important sugar crop in China and the world, which originated in tropical and subtropical areas and is a thermophilic crop. Extreme weather occurred frequently in worldwide that caused serious cold or frost damage in recent years, resulting in enormous losses in sugarcane production. Abscisic acid (ABA) regulates much important plant physiological and biochemical processes, and induces tolerance to different stresses including cold or frost damage. This experiment investigated the interrelationship between low temperature induced ABA biosynthesis and endogenous hormone balance using two sugarcane varieties, i.e. the cold tolerant variety GT 28 and cold susceptible variety YL 6. Plants were sprayed with ABA 12 h before cold treatment as opposed to the control group, where no additional substances were added. When the plants in the control group were exposed to cold stress, plant cell membranes were injured, and the GA(3) (Gibberellic acid 3) decreased, while the relative electric conductivity, MDA (Malondialdehyde), ABA, the ratio of ABA/GA(3), ratio of ABA/IAA (Indole acetic acid), and the ratio of ABA/ZR (Zeatin Riboside) all increased under the cold stress, and there are genotypic differences in response to the contents of proline, ABA and GA, and the ratio of ABA/GA exists between the sugarcane variety GT 28, cold tolerant and variety YL 6, cold susceptible under cold stress. The contents of proline and ABA and the ratio of ABA/GA are higher and the content of GA is lower in the cold tolerant variety, which is the vital physiological basis that caused two sugarcane varieties with different cold resistance. In the ABA treatment, the cell membrane injury was effectively alleviated and the contents of MDA and GA(3) decreased, but the contents of proline, ABA, and the ratio of ABA/GA(3) increased. The decreasing contents of MDA and GA(3), in contrast with the increasing contents of proline, ABA, and ratio of ABA/GA(3) in sugarcane leaves from the ABA treatment groups, were important factors that can effectively increase cold stress tolerance in sugarcane plants.