DETERMINING ENERGY-EXPENDITURE IN PRETERM INFANTS - COMPARISON OF (H2O)-H-2-O-18 METHOD AND INDIRECT CALORIMETRY
DETERMINING ENERGY-EXPENDITURE IN PRETERM INFANTS - COMPARISON OF (H2O)-H-2-O-18 METHOD AND INDIRECT CALORIMETRY
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DOI：
10.1152/ajpregu.1992.263.3.r685
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发表时间：
1992-09-01
影响因子：
--
通讯作者：
MOON, JK
中科院分区：
文献类型：
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作者：
JENSEN, CL;BUTTE, NF;MOON, JK
确定早产儿的能量消耗：(H2O)-H-2-O-18 法与间接量热法的比较。是。 J.生理学。 263 (Regulatory Integrative Comp. Physiol. 32)：R685-R692，1992。-用于估算总能量消耗 (EE(Total)) 的双标记水 (H2O)-H-2-O-18 方法对早产儿的分析误差特别敏感，因为早产儿的体内水分百分比很高，而且水通量与二氧化碳产生的比率也很高。为了评估该方法的进一步使用，通过间接量热法和(H2O)-H-2-O-18同时连续测量12名早产儿的EE，持续5天。婴儿初始体重、年龄和受孕后年龄分别为（平均值+/-SD）1,674+/-173克、4.4+/-2.6周和34.6+/-1.6周。间接热量计系统包括空气温度控制室和心率监测器。在 85.6 +/- 4.7% 的研究时间内通过间接量热法测量 EE，并在 14.4 +/- 4.7% 的研究时间内通过心率对 EE 的线性回归进行估计。 (H2O)-H-1-O-18 方法的初始剂量为 100 mg (H2O)-H-2 和 250 mg O-18/kg，最终剂量为 75 Mg O-18/kg；每天收集两次尿液。 H-2 和 O-18 富集度通过气体同位素比质谱法测量。根据测量的 H-2 和 O-18 稀释空间 (N(H)、N(O))、周转率 (k(H)、k(O)) 和测量的呼吸商计算 EE。 H-2与O-18稀释空间的比率为1.01+/-0.01并且k(O)与k(H)的比率为1.16+/-0.04。尽管方法的个体差异很大，但 (H2O)-H-2-O-18 和间接量热法对 EE 的估计一致在 1% 以内。
Determining energy expenditure in preterm infants: comparison of (H2O)-H-2-O-18 method and indirect calorimetry. Am. J. Physiol. 263 (Regulatory Integrative Comp. Physiol. 32): R685-R692, 1992.-The doubly labeled water (H2O)-H-2-O-18 method used to estimate total energy expenditure (EE(Total)) is particularly sensitive to analytic error in preterm infants, because of their high percentage of body water and the high ratio of water flux to CO2 production. To evaluate further use of this method, the EE of 12 preterm infants was measured by indirect calorimetry and (H2O)-H-2-O-18 simultaneously and continuously for 5 days. Initial infant weight, age, and postconceptional age were (means +/-SD) 1,674 +/- 173 g, 4.4 +/- 2.6 wk, and 34.6 +/- 1.6 wk, respectively. The indirect calorimeter system included an air-temperature-controlled chamber and heart rate monitor. EE was measured by indirect calorimetry for 85.6 +/- 4.7% of study time and estimated from the linear regression of heart rate on EE for 14.4 +/- 4.7% of study time. The (H2O)-H-1-O-18 method entailed an initial dose of 100 mg (H2O)-H-2 and 250 mg O-18/kg and a final dose of 75 Mg O-18/kg; urine was collected twice daily. H-2 and O-18 enrichments were measured by gas-isotope-ratio mass spectrometry. EE was calculated from measured H-2 and O-18 dilution spaces (N(H), N(O)), turnover rates (k(H), k(O)), and measured respiratory quotient. The ratio of H-2 to O-18 dilution spaces was 1.01 +/-0.01 and the ratio of k(O) to k(H) was 1.16 +/- 0.04. Estimation of EE from (H2O)-H-2-O-18 and indirect calorimetry agreed within 1%, although individual variability in methods was large.