Visualization of phosphatidylcholine, lysophosphatidylcholine and sphingomyelin in mouse tongue body by matrix-assisted laser desorption/ionization imaging mass spectrometry
Visualization of phosphatidylcholine, lysophosphatidylcholine and sphingomyelin in mouse tongue body by matrix-assisted laser desorption/ionization imaging mass spectrometry
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DOI：
10.1007/s00216-011-4924-4
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发表时间：
2011-06-01
影响因子：
4.3
通讯作者：
Zaima, Nobuhiro
中科院分区：
文献类型：
--
作者：
Enomoto, Hirofumi;Sugiura, Yuki;Zaima, Nobuhiro
哺乳动物的舌头是食物摄取过程中最重要的器官之一，因为它有助于咀嚼和吞咽。此外，舌头表面也有味觉感受器。脂类是舌头中仅次于水的第二丰富的生物分子。磷脂酰胆碱(PC)、溶血磷脂酰胆碱(LPC)和鞘磷脂(SM)等脂类被认为在细胞信号转导中起重要作用。成像质谱仪(IMS)是确定和可视化脂肪在解剖组织切片上分布的强大工具。在这项研究中，我们利用基质辅助激光解吸/电离(MALDI)-IMS对小鼠舌体切片中的PC、LPC和SM物种进行了鉴定和可视化。离子图谱显示，含二十二碳六烯酸(DHA)的PC、LPC、含亚油酸的PC和SM(D18：1/16：0)和含油酸的PC分别主要分布于肌肉、结缔组织、复层上皮和周围神经。此外，SM(D18：1/16：0)的分布与与味觉有关的神经组织在复层上皮中的分布相对应。本研究首次对PC、LPC和SM在小鼠舌体的定位进行了可视化研究。
The mammalian tongue is one of the most important organs during food uptake because it is helpful for mastication and swallowing. In addition, taste receptors are present on the surface of the tongue. Lipids are the second most abundant biomolecules after water in the tongue. Lipids such as phosphatidylcholine (PC), lysophosphatidylcholine (LPC) and sphingomyelin (SM) are considered to play fundamental roles in the mediation of cell signaling. Imaging mass spectrometry (IMS) is powerful tool for determining and visualizing the distribution of lipids across sections of dissected tissue. In this study, we identified and visualized the PC, LPC, and SM species in a mouse tongue body section with matrix-assisted laser desorption/ionization (MALDI)-IMS. The ion image constructed from the peaks revealed that docosahexaenoic acid (DHA)-containing PC, LPC, linoleic acid-containing PC and SM (d18:1/16:0), and oleic acid-containing PC were mainly distributed in muscle, connective tissue, stratified epithelium, and the peripheral nerve, respectively. Furthermore, the distribution of SM (d18:1/16:0) corresponded to the distribution of nerve tissue relating to taste in the stratified epithelium. This study represents the first visualization of PC, LPC and SM localization in the mouse tongue body.