Identification and expression pattern of one stress-responsive NAC gene from Solanum lycopersicum
Identification and expression pattern of one stress-responsive NAC gene from Solanum lycopersicum
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番茄胁迫响应 NAC 基因的鉴定及表达模式
DOI：
10.1007/s11033-011-0911-2
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发表时间：
2012-02-01
影响因子：
2.8
通讯作者：
Ye, Zhibiao
中科院分区：
文献类型：
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作者：
Han, Qinqin;Zhang, Junhong;Ye, Zhibiao
NAC(代表NAM、ATAF1、2和CUC2)家族基因已被发现在不同的发育过程和环境反应中发挥重要作用。从番茄品种Ailsa Craig的cDNA文库中初步鉴定并分离到一个新的NAC型转录因子SlNAC3。基因组DNA序列包含3个外显子和2个内含子，编码329个氨基酸的多肽。在SlNAC3的N端有一个植物特有的保守的NAC结构域。SLNAC3蛋白定位于洋葱流行细胞的细胞核内，在C末端有一个转录激活结构域，在不同的NAC中表现出极大的差异。系统发育分析表明，SlNAC3属于NAC蛋白家族中的OsNAC3亚组。组织表达谱分析表明，SlNAC3主要在花、果实和根中表达。盐、干旱胁迫和ABA处理抑制了SlNAC3的转录表达。这些数据表明，SlNAC3可能与环境和内源刺激相互作用，并可能通过ABA信号通路作为转录激活因子在植物响应盐和干旱胁迫时发挥作用。
NAC (for NAM, ATAF1, 2, and CUC2) family genes have been found to play an important role in diversified developmental processes and environmental responses. A new NAC-type transcription factor SlNAC3 was primarily identified and isolated from the cDNA libraries of tomato cultivar Ailsa Craig. It contains three exons and two introns within genomic DNA sequence and encodes a polypeptide of 329 amino acids. A plant-specific and conserved NAC domain is located in the N-terminus of SlNAC3. The protein SlNAC3 is subcellularly localized in the nucleus of onion epidemical cells and it has a transcriptional activation domain in the C-terminal region which shows extremely divergent among NACs. Phylogenetic analysis showed that SlNAC3 belonged to the OsNAC3 subgroup of the NAC protein family. Tissue expression profile analysis revealed that SlNAC3 was expressed mainly in flower, fruit and root. The transcription expression of SlNAC3 was inhibited by salt, drought stress and ABA treatment. These data demonstrate that SlNAC3 might interact with environmental and endogenous stimuli and probably function when plants response to salt and drought stresses through ABA signaling pathways as a transcriptional activator.