Host nutritional status as a contributory factor to the remodeling of schistosomal hepatic fibrosis
Host nutritional status as a contributory factor to the remodeling of schistosomal hepatic fibrosis
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DOI：
10.1590/s0074-02762003000700011
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发表时间：
2003-10-01
期刊：
影响因子：
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通讯作者：
Andrade, Zilton A
中科院分区：
文献类型：
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作者：
Coutinho, Eridan M;Barros, Andréia F;Andrade, Zilton A
断奶的瑞士小鼠经皮感染了30种曼氏血吸虫尾蚴，并在24周内从缺乏饮食转变为均衡饮食，反之亦然。每周通过测量生长曲线和食物摄入量来评估营养状况。采用组织学、形态计量学和生化方法对肝纤维化和肝周骨膜肉芽肿进行研究。在慢性感染后，所有喂食缺陷饮食的小鼠均未发生门脉周围“管道”纤维化。一个意想不到的发现是正常饮食的小鼠没有管道纤维化，可能与样本量有关。营养参数值较低主要是由于饮食不足，而不是感染。在转向均衡饮食后，“早期营养不良”组的肝脏/体重比更高。鸡蛋肉芽肿的体积密度和数值密度在营养不良的动物中达到最低。在营养不良的小鼠中，胶原蛋白的含量减少，而在营养充足的对照组和“晚期营养不良”（均衡饮食转向缺乏饮食）小鼠中，胶原蛋白的浓度更高，在肉芽肿中胶原蛋白沉积增加。这一发现表明，在“晚期”营养不良的动物中，胶原蛋白的降解和吸收受到干扰。因此，宿主营养丰富。地位在小鼠肝性血吸虫病结缔组织改变中起作用。
Weaning Swiss mice were percutaneously infected with 30 cercariae of Schistosoma mansoni and submitted to a shifting either from a deficient to a balanced diet or vice-versa, for 24 weeks. The nutritional status was weekly evaluated by measurements of growth curves and food intake. Hepatic fibrosis and periovular granulomas were studied by histological, morphometric and biochemical methods. All mice fed on a deficient diet failed to develop periportal "pipestem" fibrosis after chronic infection. An unexpected finding was the absence of pipestem fibrosis in mice on normal diet, probably related to the sample size. The lower values for nutritional parameters were mainly due to the deficient diet, rather than to infection. Liver/body weight ratio was higher in "early undernutrition" group, after shifting to the balanced diet. Volume density and numerical density of egg granulomas reached lowest values in undernourished animals. The amount of collagen was reduced in undernourished mice, attaining higher concentrations in well-fed controls and in "late undernutrition" (balanced diet shifted to a deficient one), where collagen deposition appeared increased in granulomas. That finding suggested interference with collagen degradation and resorption in "late" undernourished animals. Thus, host nutritional. status plays a role in connective tissue changes of hepatic schistosomiasis in mice.