Cytotoxic effect of ciprofloxacin in primary culture of rat astrocytes and protection by Vitamin E
Cytotoxic effect of ciprofloxacin in primary culture of rat astrocytes and protection by Vitamin E
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DOI：
10.1016/j.tox.2006.09.016
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发表时间：
2007-01-05
期刊：
影响因子：
4.5
通讯作者：
Hincal, Filiz
中科院分区：
文献类型：
--
作者：
Gurbay, Aylin;Gonthier, Brigitte;Hincal, Filiz
本研究旨在探讨环丙沙星(CPFX)对原代培养的大鼠星形胶质细胞可能的细胞毒性和氧化应激诱导作用。将培养的细胞与不同浓度的环磷酰胺(0.5~300 mg/L)共同孵育，用中性红和四甲基偶氮唑盐比色法测定细胞毒性。细胞存活率在NR实验中呈双相变化：CPFX在任何浓度下7h均未引起任何变化，而在其他时间段均为50 mg/L。0.5和5 mg/L的CPFX作用24 h后，细胞才出现增殖。用NR法和四甲基偶氮唑盐比色法检测，细胞培养96h后，维生素E预处理对300 mg/l CPFX的细胞毒性有完全的保护作用。用NR法检测，SOD预处理有部分保护作用，而用四甲基偶氮唑盐(MTT)法检测，则无保护作用。随着药物细胞毒性浓度的增加，细胞内脂质过氧化作用明显增强，但细胞内总谷胱甘肽含量和过氧化氢酶活性无明显变化。这项研究中获得的数据表明，与我们之前在成纤维细胞上的结果一致，CPFX诱导的细胞毒性与氧化应激有关。CPFX的双相效应可能源于活性氧、细胞增殖和细胞活力之间复杂的剂量依赖关系。(C)2006爱思唯尔爱尔兰有限公司。保留所有权利。
The aim of this study was to investigate the possible cytotoxic and oxidative stress inducing effects of ciprofloxacin (CPFX) on primary cultures of rat astrocytes. The cultured cells were incubated with various concentrations of CPFX (0.5-300 mg/l), and cytotoxicity was determined by neutral red (NR) and MTT assays. Survival profile of cells was biphasic in NR assay: CPFX did not cause any alteration at any concentration for 7 h, whereas = 50 mg/l CPFX exposure in all other time periods. Cell proliferation was only seen in 24 h of incubation with 0.5 and 5 mg/l CPFX. Vitamin E pretreatment of cell cultures were found to be providing complete protection against cytotoxicity of 300 mg/1 CPFX in 96 h incubation when measured with both NR and MTT assays. The SOD pretreatment was partially protective with NR assay, but no protection was noted when measured with MTT. A significant enhancement of lipid peroxidation was observed with the cytotoxic concentration of the drug, but total glutathione content and catalase activity of cells did not change. The data obtained in this study suggest that, in accordance with our previous results with fibroblast cells, CPFX-induced cytotoxicity is related to oxidative stress. And the biphasic effect of CPFX possibly resulted from the complex dose-dependent relationships between reactive oxygen species, cell proliferation, and cell viability. (c) 2006 Elsevier Ireland Ltd. All rights reserved.