Phosphorylation of phosphoenolpyruvate carboxykinase (PEPCK) and phosphoenolpyruvate carboxylase (PEPC) in the flesh of fruits.
Phosphorylation of phosphoenolpyruvate carboxykinase (PEPCK) and phosphoenolpyruvate carboxylase (PEPC) in the flesh of fruits.
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DOI：
10.1016/j.plaphy.2016.07.021
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发表时间：
2016-11
期刊：
影响因子：
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通讯作者：
R. P. Walker;A. Paoletti;R. Leegood;F. Famiani
中科院分区：
文献类型：
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作者：
R. P. Walker;A. Paoletti;R. Leegood;F. Famiani
本研究确定了一系列水果果肉中磷酸烯醇式丙酮酸羧化酶（PEPCK）和磷酸烯醇式丙酮酸羧化酶（PEPC）是否被磷酸化。这是通过将果肉与32 P [P]（其中32 P [P] = 32 PO 43-）孵育来完成的，然后使用特异性抗血清从提取物中免疫沉淀PEPCK和PEPC。然后通过SDS-PAGE凝胶的放射自显影测定32 P [P]掺入这些酶中。这两种酶在葡萄、番茄、樱桃和李的果肉中均发生了磷酸化。PEPCK在葡萄种子发育过程中也发生了磷酸化。PEPCK的蛋白水解切割显示其在位于其N-末端延伸的位点处被磷酸化。这些酶的潜在磷酸化可能有助于协调调节其在果实果肉和发育种子中的活性。
This study determined whether phosphoenolpyruvate carboxykinase (PEPCK) and phosphoenolpyruvate carboxylase (PEPC) are phosphorylated in the flesh of a range of fruits. This was done by incubating fruit flesh with32P[P] (where32P[P] =32PO43−), then PEPCK and PEPC were immunoprecipitated from extracts using specific antisera. The incorporation of32P[P] into these enzymes was then determined by autoradiography of SDS-PAGE gels. Both enzymes were subject to phosphorylationin vivoin the flesh of grape, tomato, cherry and plum. PEPCK was also subject to phosphorylationin vivoin developing grape seeds. Proteolytic cleavage of PEPCK showed that it was phosphorylated at a site(s) located on its N-terminal extension. Potentially phosphorylation of these enzymes could contribute to the coordinate regulation of their activities in the flesh of fruits and in developing seeds.