Regulation of 2D DNA Nanostructures by the Coupling of Intrinsic Tile Curvature and Arm Twist
Regulation of 2D DNA Nanostructures by the Coupling of Intrinsic Tile Curvature and Arm Twist
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DOI：
10.1021/jacs.1c13601
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发表时间：
2022-04-20
影响因子：
15
通讯作者：
Xiao, Shou-Jun
中科院分区：
文献类型：
--
作者：
Jiang, Chuan;Lu, Biao;Xiao, Shou-Jun
交叉点之间DNA双链的过度缠绕和欠缠绕已分别用于设计左手和右手DNA折纸纳米结构。对于从自组装瓦片获得的DNA管，先前仅使用瓦片的固有曲率的理论方法来解释它们的形成。关于DNA纳米结构中瓦片的固有曲率的定量和结构描述的细节迄今为止从未得到解决。在这项工作中，我们设计了三种类型的瓷砖核心围绕一个圆形支架使用三个和四个分支的路口。用具有两种瓦片间距离（奇数和偶数个DNA半圈）的臂连接瓦片核心倾向于分别形成平面2D晶格和管。结合生物素的链霉亲和素被用作标记技术来表征管的内表面和外表面，从而表征具有可寻址面的二面体的瓦片构象。通过瓦片的固有曲率与臂扭曲的耦合，获得具有右手或左手手性的DNA管。我们能够分配的手性指数（n，m）的管，其结构解决了原子力显微镜在单瓦水平，因此估计的整体曲率的管（或其组件瓦）使用正多边形模型，近似其横截面。更深入地了解不同类型的扭转力对DNA管的综合作用将非常有助于未来设计更精细的DNA纳米结构。
The overwinding and underwinding of DNA duplexes between junctions have been used in designing left- and right-handed DNA origami nanostructures, respectively. For DNA tubes obtained from self-assembled tiles, only a theoretical approach of the intrinsic curvature of the tiles has been previously used to explain their formation. Details regarding the quantitative and structural descriptions of the tile's intrinsic curvature in DNA nanostructures have so far never been addressed. In this work, we designed three types of tile cores built around a circular scaffold using three- and four-branched junctions. Joining the tile cores with arms having two kinds of inter-tile distances, an odd and an even number of DNA half-turns, tended to form planar 2D lattices and tubes, respectively. Streptavidin bound to biotin was used as a labeling technique to characterize the inside and outside surfaces of the tubes and thereby the tile conformation of dihedrals with addressable faces. DNA tubes with either right- or left-handed chirality were obtained by the coupling of the intrinsic curvature of the tiles with the arm twist. We were able to assign the chiral indices (n,m) to a tube with its structure resolved by AFM at the single-tile level and therefore to estimate the global curvature of the tube (or its component tile) using a regular polygon model that approximated its transverse section. A deeper understanding of the integrated actions of different types of twisting forces on DNA tubes will be extremely helpful in engineering more elaborate DNA nanostructures in the future.