Intranasal delivery of cells to the brain
Intranasal delivery of cells to the brain
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DOI：
10.1016/j.ejcb.2009.02.001
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发表时间：
2009-06-01
影响因子：
6.6
通讯作者：
Frey, William H., II
中科院分区：
文献类型：
--
作者：
Danielyan, Lusine;Schaefer, Richard;Frey, William H., II
对于神经退行性疾病，基于细胞的疗法的安全性和有效性取决于细胞给药的模式。我们假设鼻内给药的细胞可以绕过血脑屏障，从鼻粘膜穿过筛板沿着嗅觉神经通路迁移到大脑和脑脊液（CSF）中。这将最大限度地减少或消除细胞移植物向周围器官的分布，并将有助于免除神经外科细胞植入。在这里，我们展示了将荧光标记的大鼠间充质干细胞（MSC）或人神经胶质瘤细胞鼻内应用到幼鼠和大鼠后，细胞经鼻递送至大脑。细胞穿过筛板后，确定了两条迁移途径：（1）迁移到嗅球和大脑的其他部分：（2）进入脑脊液，沿着皮质表面运动，然后进入脑实质。在应用细胞前30分钟鼻内应用透明质酸酶处理，增强了细胞的递送。鼻内递送为细胞递送至中枢神经系统提供了一种新的非侵入性方法。 (C) 2009 爱思唯尔有限公司。版权所有。
The safety and efficacy of cell-based therapies For neurodegenerative diseases depends on the mode of cell administration. We hypothesized that intranasally administered cells could bypass the blood-brain barrier by migrating from the nasal mucosa through the cribriform plate along the olfactory neural pathway into the brain and cerebrospinal fluid (CSF). This would minimize or eliminate the distribution of cellular grafts to peripheral organs and will help to dispense with neurosurgical cell implantation. Here we demonstrate transnasal delivery of cells to the brain Following intranasal application of fluorescently labeled rat mesenchymal stem cells (MSC) or human glioma cells to naive mice and rats. After cells crossed the cribriform plate, two migration routes were identified: (1) migration into the olfactory bulb and to other parts of the brain: (2) entry into the CSF with movement along the surface of the cortex followed by entrance into the brain parenchyma. The delivery of cells was enhanced by hyaluronidase treatment applied intranasally 30 min prior to the application of cells. Intranasal delivery provides a new non-invasive method For cell delivery to the CNS. (C) 2009 Elsevier GmbH. All rights reserved.