Escherichia coli Adhesion and Biofilm Formation on Polydimethylsiloxane are Independent of Substrate Stiffness
Escherichia coli Adhesion and Biofilm Formation on Polydimethylsiloxane are Independent of Substrate Stiffness
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DOI：
10.1021/acs.langmuir.0c00130
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发表时间：
2021-01-12
期刊：
影响因子：
3.9
通讯作者：
Allain, Jean Paul
中科院分区：
文献类型：
--
作者：
Arias, Sandra L.;Devorkin, Joshua;Allain, Jean Paul
生物医学设备表面的细菌粘附和生物膜形成是有害过程，会损害患者安全和材料功能。生物膜生长涉及多种物理化学因素，包括表面特性。其中，材料刚度最近被认为会影响各种聚合物和水凝胶中的微生物粘附和生物膜生长。然而，关于材料刚度在生物膜形成中的作用以及非常顺应的基质是否对细菌细胞粘附有害，尚无明确的共识。在这里，通过系统地调整基材形貌和刚度，同时保持聚二甲基硅氧烷基材的表面自由能恒定，我们表明微米和亚微米尺度的形貌图案赋予表面独特的性能，而与材料刚度无关。目前的工作提供了对材料刚度在细菌生理学中的作用的更好理解，并且可能构成一种经济有效且简单的策略，以减少细菌附着和生物膜生长，即使在非常顺应和疏水的聚合物中也是如此。
Bacterial adhesion and biofilm formation on the surface of biomedical devices are detrimental processes that compromise patient safety and material functionality. Several physicochemical factors are involved in biofilm growth, including the surface properties. Among these, material stiffness has recently been suggested to influence microbial adhesion and biofilm growth in a variety of polymers and hydrogels. However, no clear consensus exists about the role of material stiffness in biofilm initiation and whether very compliant substrates are deleterious to bacterial cell adhesion. Here, by systematically tuning substrate topography and stiffness while keeping the surface free energy of polydimethylsiloxane substrates constant, we show that topographical patterns at the micron and submicron scale impart unique properties to the surface which are independent of the material stiffness. The current work provides a better understanding of the role of material stiffness in bacterial physiology and may constitute a cost-effective and simple strategy to reduce bacterial attachment and biofilm growth even in very compliant and hydrophobic polymers.