Transcriptional regulation of the Oct4 gene, a master gene for pluripotency.
Transcriptional regulation of the Oct4 gene, a master gene for pluripotency.
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DOI：
10.14670/hh-25.405
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发表时间：
2010-03
影响因子：
2
通讯作者：
Kikyo N
中科院分区：
文献类型：
--
作者：
Kellner S;Kikyo N
Oct4是维持人类和小鼠胚胎干（ES）细胞以及早期胚胎细胞的未分化状态（自我更新）和多能性所需的最重要的转录因子之一。此外，Oct4是唯一已知的必须外源引入分化细胞以产生诱导多能干细胞（iPS）的转录因子。因此，了解Oct4在ES细胞和胚胎中的转录调控以及它在iPS细胞形成过程中如何被激活是非常重要的。本文主要从DNA甲基化、孤儿核受体结合、组蛋白修饰以及与其他多能性因子的协同作用等方面对小鼠Oct4基因的调控进行综述。我们还将提出几个需要在未来工作中解决的关键问题，以提高我们对Oct4基因调控及其在自我更新和多能性中的重要作用的理解。
Oct4 is one of the most important transcription factors required to maintain an undifferentiated state (self-renewal) and pluripotency of human and mouse embryonic stem (ES) cells as well as early embryonic cells. In addition, Oct4 is the only known transcription factor that has to be exogenously introduced into differentiated cells to make induced pluripotent stem (iPS) cells. Therefore, it is of great importance to understand how Oct4 transcription is regulated in ES cells and embryos and how it becomes activated during iPS cell formation. In this article, we will review the regulation of the mouse Oct4 gene from the viewpoint of DNA methylation, binding of orphan nuclear receptors, histone modifications and synergistic effects with other pluripotency factors. We will also raise several key questions that need to be addressed in future work to improve our understanding of Oct4 gene regulation and its essential role in self-renewal and pluripotency.