A heteropolysaccharide, L-fuco-D-manno-1,6-α-D-galactan extracted from Grifola frondosa and antiangiogenic activity of its sulfated derivative
A heteropolysaccharide, L-fuco-D-manno-1,6-α-D-galactan extracted from Grifola frondosa and antiangiogenic activity of its sulfated derivative
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从灰树花中提取的杂多糖 L-岩藻糖-D-甘露糖-1,6-α-D-半乳聚糖及其硫酸化衍生物的抗血管生成活性
DOI：
10.1016/j.carbpol.2013.09.085
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发表时间：
2014-01-30
影响因子：
11.2
通讯作者：
Ding, Kan
中科院分区：
文献类型：
--
作者：
Wang, Ying;Shen, Xiaokun;Ding, Kan
肿瘤的生长和转移依赖于血管生成，因此阻断血管生成是治疗癌症的有希望的方法。本文报道了从灰树花子实体中分离得到的一种新型水溶性多糖GFPW的结构和生物学特性。化学和光谱分析表明，GFPW的平均分子量为15.7 kDa，具有由α-1，6-连接的吡喃半乳糖残基组成的主链，与α-1，3-连接的岩藻糖残基和α-末端甘露糖的O-2连接的分支。采用氯磺酸-吡啶法合成了GFPW的硫酸化衍生物Sul-GFPW，取代度为0.33。根据C-13 NMR谱，推断取代位置在C-2和C-3。体外血管生成实验表明，Sul-GFPW能显著抑制内皮细胞增殖，并呈剂量和时间依赖性，同时能减少内皮细胞迁移和管腔形成。(C)2013爱思唯尔有限公司保留所有权利。
The tumor growth and metastasis are angiogenesis dependent, thus blockade of angiogenesis is a promising approach for treatment of cancer. Herein we reported the structural and biological features of a novel water-soluble polysaccharide named GFPW from the fruit body of Grifola frondosa. Chemical and spectral analysis revealed that GFPW with an average molecular weight of 15.7 kDa, possessed a backbone consisting of alpha-1,6-linked galactopyranosyl residues, with branches attached to O-2 of alpha-1,3-linked fucose residues and alpha-terminal mannose. By the chlorosulfonic acid-pyridine method, we prepared a sulfated derivative of GFPW, Sul-GFPW, with a substitution degree of 0.33. According to the C-13 NMR spectrum, the substitution position was deduced at C-2 and C-3. The angiogenesis assays in vitro showed that Sul-GFPW significantly inhibited endothelial cell proliferation in a dose- and time-dependent manner, and reduced endothelial cell migration and tube formation as well. (C) 2013 Elsevier Ltd. All rights reserved.