COMPARATIVE GENOMIC HYBRIDIZATION FOR MOLECULAR CYTOGENETIC ANALYSIS OF SOLID TUMORS
COMPARATIVE GENOMIC HYBRIDIZATION FOR MOLECULAR CYTOGENETIC ANALYSIS OF SOLID TUMORS
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DOI：
10.1126/science.1359641
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发表时间：
1992-10-30
期刊：
影响因子：
56.9
通讯作者：
PINKEL, D
中科院分区：
文献类型：
--
作者：
KALLIONIEMI, A;KALLIONIEMI, OP;PINKEL, D
比较基因组杂交产生DNA序列拷贝数的图谱，作为整个基因组中染色体位置的函数。差异标记的测试DNA和正常参考DNA同时与正常染色体扩散杂交。用两种不同的荧光染料检测杂交。DNA序列的增加或丢失区域，如缺失、重复或扩增，被视为沿靶染色体沿着的两种荧光染料的强度比率的变化。肿瘤细胞系和原发性膀胱肿瘤的分析确定了16个不同的扩增区域，许多在以前不知道被扩增的基因座中。
Comparative genomic hybridization produces a map of DNA sequence copy number as a function of chromosomal location throughout the entire genome. Differentially labeled test DNA and normal reference DNA are hybridized simultaneously to normal chromosome spreads. The hybridization is detected with two different fluorochromes. Regions of gain or loss of DNA sequences, such as deletions, duplications, or amplifications, are seen as changes in the ratio of the intensities of the two fluorochromes along the target chromosomes. Analysis of tumor cell lines and primary bladder tumors identified 16 different regions of amplification, many in loci not previously known to be amplified.