Phylogenetic analysis of Ganoderma based on nearly complete mitochondrial small-subunit ribosomal DNA sequences
Phylogenetic analysis of Ganoderma based on nearly complete mitochondrial small-subunit ribosomal DNA sequences
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DOI：
10.1080/15572536.2005.11832922
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发表时间：
2004-07
期刊：
影响因子：
2.8
通讯作者：
S. Hong;H. Jung
中科院分区：
文献类型：
--
作者：
S. Hong;H. Jung
为研究灵芝属植物的系统发育，对灵芝线粒体SSU rDNA的特征和结构进行了研究。系统发育信息主要集中在V1、V4、V5、V6和V9可变区，但保守区中的信息位点对灵芝类群间系统发育关系的解析也有显著贡献。可变结构域的二级结构信息被认为是一个有用的标记，在划分系统发育组。本研究中所用的灵芝菌株被分为六个单系群。灵芝属（Ganoderma）是灵芝属（Ganoderma）的一个独特的基本分支，与其它灵芝属（Ganoderma）和为G. colosus，似乎是一个有效的属。树舌（Ganoderma applanatum）和G.裂叶藓属Elfvingia亚属为单系群。北美的松杉灵芝（Ganoderma tsugae）和北美的松杉灵芝（G.来自欧洲的valesiacum，两者都生活在针叶树上，关系密切。俄勒冈灵芝（Ganoderma oregonense）和G. lucidum与G. tsugae和G. valesiacum。标记为G.产于美国和台湾硬木上的灵芝（G. lucidum）与产于美国和台湾硬木上的灵芝（G. lucidum）聚为一类。resinaceum，G. pfeifferi和G.亚安波因原变种laevisporum，它们都产生厚垣孢子。两株G. lucidum和G. resinaceum为同一种。标记为G.韩国和日本的灵芝菌株是单系的，与G.产于欧洲和北美的灵芝。寄主关系和厚垣孢子在培养物中的存在被证明是灵芝系统学和系统发育关系的重要特征。
Characteristics and structures of mt SSU rDNA were investigated for the phylogenetic study of Ganoderma. Phylogenetic information was concentrated mostly in the V1, V4, V5, V6 and V9 variable domains, but informative sites in conserved domains also significantly contributed in resolving phylogenetic relationships between Ganoderma groups. Secondary structure information of variable domains was found to be a useful marker in delineation of phylogenetic groups. Strains of Ganoderma species used in this study were divided into six monophyletic groups. Ganoderma colossus made a distinct basal lineage from other Ganoderma species and Tomophagus, created for G. colosuss, appeared to be a valid genus. Ganoderma applanatum and G. lobatum classified in subgenus Elfvingia made a monophyletic group. Ganoderma tsugae from North America and G. valesiacum from Europe, both living on conifers, were closely related. Ganoderma oregonense and strains labeled G. lucidum from Europe and Canada were grouped with G. tsugae and G. valesiacum. Strains labeled G. lucidum living on hardwoods from the United States and Taiwan were grouped with G. resinaceum, G. pfeifferi and G. subamboinense var. laevisporum, and they all produced chlamydospores. Two strains labeled G. lucidum and three strains labeled G. resinaceum from America were concluded to be conspecific. Strains labeled G. lucidum from Korea and Japan were monophyletic and were distinguished from strains labeled G. lucidum from Europe and North America. Host relationships and the presence of chlamydospores in culture proved to be important characteristics in the systematics as well as the phylogenetic relationships of Ganoderma.