Proteomics - Proteomics in genomeland
Proteomics - Proteomics in genomeland
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DOI：
10.1126/science.291.5507.1221
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发表时间：
2001-02-16
期刊：
影响因子：
56.9
通讯作者：
Fields, S
中科院分区：
文献类型：
--
作者：
Fields, S
If the architect you hired to design your home brought you a blueprint that solely consisted of a long list of parts that began “windowwabeborogovestaircasedoorjubjub…,” you might start to wonder if and when you will see your new house. Some people have similar reservations about the recently “completed” human genome sequence, heralded as the “genetic blueprint” that will revolutionize biology and medicine. Deciphering how a mere 10 7 nucleotides result in a yeast cell—let alone how 3× 10 9 nucleotides result in Tiger Woods or Britney Spears—cannot begin until the genes have been annotated. This step includes figuring out the proteins that these genes encode and what they do for a living. But understanding how all of these proteins collaborate to carry out cellular processes is the real enterprise at hand.In the wonderland of complete sequences, there is much that genomics cannot do, and so the future belongs to proteomics: the analysis of complete complements of proteins. Proteomics includes not only the identification and quantification of proteins, but also the determination of their localization, modifications, interactions, activities, and, ultimately, their function. Initially encompassing just two-dimensional (2D) gel electrophoresis for protein separation and identification, proteomics now refers to any procedure that characterizes large sets of proteins. The explosive growth of this field is driven by multiple forces—genomics and its revelation of more and more new proteins; powerful protein technologies, such as newly developed mass spectrometry approaches, global two-hybrid techniques, and spin-offs from DNA arrays; and innovative computational tools and methods to process, analyze, and interpret prodigious amounts of data.
