Genome-wide Discovery of Circular RNAs in the Leaf and Seedling Tissues of Arabidopsis Thaliana.
Genome-wide Discovery of Circular RNAs in the Leaf and Seedling Tissues of Arabidopsis Thaliana.
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DOI：
10.2174/1389202918666170307161124
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发表时间：
2017-08
期刊：
影响因子：
2.6
通讯作者：
Zhang C
中科院分区：
文献类型：
--
作者：
Dou Y;Li S;Yang W;Liu K;Du Q;Ren G;Yu B;Zhang C
最近，随着RNA-seq技术的发展，在哺乳动物细胞中进行了环状RNA的鉴定和功能研究，环状RNA是一种由线性RNA分子的3'和5'末端连接产生的非编码RNA。 与动物相比，植物中环状RNA的研究还不够深入，因此利用我们自己开发的生物信息学工具，对拟南芥中的几个非编码RNA的RNA-seq数据集进行了全基因组范围的环状RNA候选物鉴定。 从RNA-seq数据中鉴定了总共164种环状RNA候选物，并且实验验证了4种环状RNA转录物，包括外显子和内含子环状RNA。有趣的是，我们的研究结果表明，环状RNA转录物在叶片组织的光合作用系统中富集，并与其亲本基因的较高表达水平相关。在所有40个具有环状RNA候选者的基因中，有16个与光合作用系统相关，并且在总共146个外显子环状RNA候选者中，有63个在叶绿体中被发现。
Recently, identification and functional studies of circular RNAs, a type of non-coding RNAs arising from a ligation of 3’ and 5’ ends of a linear RNA molecule, were conducted in mammalian cells with the development of RNA-seq technology. Since compared with animals, studies on circular RNAs in plants are less thorough, a genome-wide identification of circular RNA candidates in Arabidopsis was conducted with our own developed bioinformatics tool to several existing RNA-seq datasets specifically for non-coding RNAs. A total of 164 circular RNA candidates were identified from RNA-seq data, and 4 circular RNA transcripts, including both exonic and intronic circular RNAs, were experimentally validated. Interestingly, our results show that circular RNA transcripts are enriched in the photosynthesis system for the leaf tissue and correlated to the higher expression levels of their parent genes. Sixteen out of all 40 genes that have circular RNA candidates are related to the photosynthesis system, and out of the total 146 exonic circular RNA candidates, 63 are found in chloroplast.
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影响因子：
14.9
作者：
Danan M;Schwartz S;Edelheit S;Sorek R
通讯作者：
Sorek R
影响因子：
4.5
作者：
Burd CE;Jeck WR;Liu Y;Sanoff HK;Wang Z;Sharpless NE
通讯作者：
Sharpless NE
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
64.5
作者：
HALBREICH, A;PAJOT, P;SLONIMSKI, P
通讯作者：
SLONIMSKI, P
水稻环状RNA的全转录组研究
DOI：
10.1261/rna.052282.115
发表时间：
2015-12
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Lu T;Cui L;Zhou Y;Zhu C;Fan D;Gong H;Zhao Q;Zhou C;Zhao Y;Lu D;Luo J;Wang Y;Tian Q;Feng Q;Huang T;Han B
通讯作者：
Han B