Collagenase-1 (MMP-1) activity in equine synovial fluid: influence of age, joint pathology, exercise and repeated arthrocentesis
Collagenase-1 (MMP-1) activity in equine synovial fluid: influence of age, joint pathology, exercise and repeated arthrocentesis
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DOI：
10.2746/0425164044864705
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发表时间：
2004-01-01
影响因子：
2.2
通讯作者：
Van Weeren, PR
中科院分区：
文献类型：
--
作者：
Brama, PAJ;Van den Boom, R;Van Weeren, PR
进行研究的原因：基质金属蛋白酶（MMPs）被认为是生理和病理组织重塑的候选生物标志物，因为它们在关节软骨稳态中起关键作用。由于胶原结构的破坏被认为是慢性退行性疾病如骨关节炎（OA）的关键，胶原酶形成了一个有趣的MMP亚群。只有当已知并考虑到生理过程（如发育和生长）以及外部影响和干预（如运动和反复关节穿刺）引起的模式波动时，才能正确评估滑液（SF）中任何生物标志物的意义。目的：研究MMP-1在不同发育阶段的马SF中的活性以及在受OA影响的关节中的活性，以及运动和反复关节穿刺对其的影响。MMP-1的活性测定在SF的胎儿，青少年和成熟的马的正常关节，并在SF的马患有OA，使用内部淬灭的荧光肽底物。MMP-1的活性也进行了测量SF从马进行运动方案和那些进行重复arthrocentesis.Results：与年龄相关的SF活性MMP-1的水平下降进行了观察。MMP-1的活性是15倍，在胎儿比在幼年动物，这表明显着较高的MMP-1活性水平比成熟的马。在OA关节的SF中，MMP-1活性增加。运动并没有影响MMP-1活性SF，但反复arthrocentesis（60小时内）增加MMP-1 activitysignificant.Conclusions：高MMP-1活性SF的年轻人平行的高代谢活动发生在快速增长和分化的早期年龄。OA关节SF中MMP-1活性升高可能反映了病理性基质降解，证实了MMP-1作为早期关节疾病生化标志物的潜力。适度的运动是不太可能影响结果的MMP-1活性测量马SF，但arthrocentesis应考虑作为一个可能的混淆factor.Potential相关性：鉴于至关重要的作用，胶原基质的组织完整性，MMP-1的活性可能是一个有用的工具，在诊断，治疗或预后研究马怀疑OA。然而，应注意排除MMP-1活性的波动诱导的生理过程，如发展和增长，并通过干预措施，如反复关节穿刺。
Reasons for performing study: Matrix metalloproteinases (MMPs) are considered candidate biomarkers for both physiological and pathological tissue remodelling because of their key role in articular cartilage homeostasis. As disruption of the collagenous architecture is thought to be pivotal in chronic degenerative diseases such as osteoarthritis (OA), the collagenases form an interesting subset of the MMPs. The significance of any biomarker in synovial fluid (SF) can be assessed properly only when fluctuations in patterns induced by physiological processes such as development and growth, and by external influences and interventions such as exercise and repeated arthrocentesis, are known and taken into account.Objectives: To investigate the activity of MMP-1 in equine SF at different stages of development and in joints affected by OA, and the influence of exercise and repeated arthrocentesis thereon.Methods: MMP-1 activity was determined in SF of normal joints of fetal, juvenile and mature horses, and in SF of horses suffering from OA, using an internally quenched fluorogenic peptide substrate. MMP-1 activity was also measured in SF from horses subjected to an exercise regimen and those subjected to repeated arthrocentesis.Results: An age-related decline in the SF levels of active MMP-1 was observed. MMP-1 activity was 15-fold higher in fetal than in juvenile animals, which showed significantly higher MMP-1 activity levels than mature horses. In SF of OA joints, MMP-1 activity was increased. Exercise did not affect MMP-1 activity in SF, but repeated arthrocentesis (within 60 h) increased MMP-1 activity significantly.Conclusions: The high MMP-1 activity in SF of young individuals parallels the high metabolic activity occurring during rapid growth and differentiation at early age. The elevated MMP-1 activity in SF of OA joints probably reflects pathological matrix degradation, confirming the potential of MMP-1 to serve as a biochemical marker for early joint disease. Moderate exercise is not likely to influence the outcome of MMP-1 activity measurements in equine SF, but arthrocentesis should be taken into account as a possible confounding factor.Potential relevance: Given the crucial role of the collagen matrix for tissue integrity, MMP-1 activity may be a useful tool in diagnostic, therapeutic or prognostic studies in horses suspected of OA. However, care should be taken to exclude fluctuations in MMP-1 activity induced by physiological processes such as development and growth, and by interventions such as repeated arthrocentesis.