Identification of Cells Expressing IL-17A and IL-17F in the Lungs of Patients With COPD
Identification of Cells Expressing IL-17A and IL-17F in the Lungs of Patients With COPD
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DOI：
10.1378/chest.10-0779
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发表时间：
2011-05-01
期刊：
影响因子：
9.6
通讯作者：
Singh, Dave
中科院分区：
文献类型：
--
作者：
Eustace, Amanda;Smyth, Lucy J. C.;Singh, Dave
背景：淋巴细胞分泌IL-17 A和IL-17 F，其增强先天性免疫应答。尚未研究COPD小气道中的IL-17表达。本研究的目的是定量COPD患者与对照受试者相比外周肺组织中IL-17 A和IL-17 F的表达，并鉴定表达IL-17的炎性细胞。采用免疫组化法检测外周肺组织中IL-17的表达（18例COPD患者，10例吸烟者和10例肺功能正常的非吸烟者）和诱导痰（12例COPD患者和6例非吸烟者）。来自8名COPD患者，8名吸烟者和7名非吸烟者的肺泡巨噬细胞用于逆转录-聚合酶链反应（RT-PCR）analysis.Results：COPD患者小气道上皮下表达IL-17 A的炎性细胞数量高于吸烟者（P= 0.01）和非吸烟者（P= 0.02）。IL-17 A在该区域的表达高于IL-17 F。IL-17 A由淋巴细胞、中性粒细胞和巨噬细胞表达（通过RT-PCR证实）。IL-17 F的表达大于IL-17 A在上皮细胞和淋巴滤泡，虽然没有受试者groups.Conclusions之间的差异：我们的研究结果表明，不同的角色IL-17 A和IL-17 F在COPD的发病机制。IL-17 A在小气道上皮下炎症中发挥作用，而IL-17 F似乎在淋巴滤泡中发挥更突出的作用。胸部2011; 139（5）：1089-1100
Background: Lymphocytes secrete IL-17A and IL-17F, which enhance innate immune responses. IL-17 expression has not been studied in COPD small airways. The aim of this study was to quantify IL-17A and IL-17F expression in the peripheral lung tissue of patients with COPD compared with control subjects and to identify inflammatory cells that express IL-17.Methods: IL-17 expression was assessed using immunohistochemistry in peripheral lung tissue (18 patients with COPD and 10 smokers and 10 nonsmokers with normal lung function) and induced sputum (12 patients with COPD and six nonsmokers). Alveolar macrophages from eight patients with COPD, eight smokers, and seven nonsmokers were used for reverse transcriptase-polymerase chain reaction (RT-PCR) analysis.Results: The number of inflammatory cells expressing IL-17A in the small airway subepithelium was higher in patients with COPD than in smokers (P=.01) and nonsmokers (P=.02). IL-17A expression was higher than IL-17F in this region. IL-17A was expressed by lymphocytes, neutrophils, and macrophages (confirmed by RT-PCR). The expression of IL-17F was greater than IL-17A in epithelial cells and lymphoid follicles, although there were no differences among subject groups.Conclusions: Our findings indicate different roles for IL-17A and IL-17F in the pathogenesis of COPD. IL-17A plays a role in small airway subepithelial inflammation, whereas IL-17F appears to play a more prominent role within lymphoid follicles. CHEST 2011; 139(5):1089-1100