Reduction of Foxp3+T cell subsets involved in incidence of chronic graft-versus-host disease after allogeneic hematopoietic stem cell transplantation
Reduction of Foxp3+T cell subsets involved in incidence of chronic graft-versus-host disease after allogeneic hematopoietic stem cell transplantation
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DOI：
10.1002/hon.2255
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发表时间：
2017-03-01
影响因子：
3.3
通讯作者：
Huang, He
中科院分区：
文献类型：
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作者：
Hu, Yongxian;Cui, Qu;Huang, He
Foxp 3 + T细胞（CD 4 + Treg和CD 8 + Treg）已被证明在异基因造血干细胞移植（Allo-HSCT）后维持耐受中发挥作用。我们发现Foxp 3 + TCR+ Treg细胞（TCRs）具有调节功能。在本研究中，招募患者并将其分为非cGVHD、限制性cGVHD和广泛性cGVHD组。健康志愿者作为健康组。通过流式细胞术评估Treg细胞。ELISA法检测血清中IL-2、肿瘤坏死因子-、干扰素-和转化生长因子-1（TGF-1）的水平。结果显示，非cGVHD组CD 4 + T细胞亚群、CD 8 + T细胞亚群和T细胞亚群均显著高于健康组、局限性cGVHD组和广泛性cGVHD组。与广泛型cGVHD组相比，局限型cGVHD组上述三种类型的THBVDNA的比例均显著升高。非cGVHD组TGF-1水平明显高于其他各组。斯皮尔曼相关分析显示TGF-1和IL-2水平的升高与Treg亚群的增加呈正相关，表明TGF-1和IL-2参与了Foxp 3 + Treg在体内的扩增过程。我们的研究结果支持增加异基因造血干细胞移植后的T细胞数量将是控制cGVHD的首选策略。版权所有（c）2015约翰威利父子有限公司
Foxp3+ T cells (CD4+ Tregs and CD8+ Treg) have been demonstrated to play roles in the maintenance of tolerance after allogeneic hematopoietic stem cell transplantation (Allo-HSCT). We have found that Foxp3+ TCR+ Treg cells (Tregs) exerted regulatory functions. In the current study, patients were recruited and divided as non-cGVHD, limited cGVHD and extensive cGVHD groups. Healthy volunteers were recruited as healthy group. Treg cells were evaluated by flow cytometry. Serum cytokine levels of IL-2, tumour necrosis factor-, interferon- and transforming growth factor-1 (TGF-1) were evaluated by ELISA. The results showed that percentages of CD4+ Tregs, CD8+ Tregs and Tregs were all significantly increased in non-cGVHD group compared with those in healthy group, limited cGVHD group and extensive cGVHD group. Moreover, compared with extensive cGVHD group, percentages of these three types of Tregs were significantly increased in limited cGVHD group. The levels of TGF-1 increased dramatically in non-cGVHD group compared with other groups. Spearman's correlation analysis revealed that the increased levels of TGF-1 and IL-2 were positively associated with increased Treg subsets, indicating that TGF-1 and IL-2 participated in the expansion process of Foxp3+ Tregs in vivo. Our findings support that increasing the number of Tregs following allo-HSCT would be a preferential strategy for controlling cGVHD. Copyright (c) 2015 John Wiley & Sons, Ltd.