Profiles of IgE Sensitization to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20 in Korean House Dust Mite Allergy Patients.
Profiles of IgE Sensitization to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20 in Korean House Dust Mite Allergy Patients.
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DOI：
10.4168/aair.2015.7.5.483
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发表时间：
2015-09
期刊：
影响因子：
--
通讯作者：
Park JW
中科院分区：
文献类型：
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作者：
Jeong KY;Lee JY;Son M;Yi MH;Yong TS;Shin JU;Lee KH;Kim YJ;Park KH;Park HJ;Lee JH;Park JW
测定纯化的屋尘螨（HDM）过敏原特异性IgE可提高过敏诊断水平。本研究旨在研究患有呼吸道过敏和特应性皮炎（AD）的韩国HDM过敏受试者对Der f 1、Der f 2、Der f 6、Der f 8、Der f 10和Der f 20的致敏特征。重组HDM变应原在巴斯德毕赤酵母（Der f 1）或大肠杆菌（5种变应原）中产生。通过ELISA评估对单个重组变应原和螨总提取物的IgE反应性。Der f 1、Der f 2、Der f 6、Der f 8、Der f 10和Der f 20的阳性率分别为79.1%、79.1%、9.3%、6.2%、6.6%和94.7%。Der f1和Der f2联合检测的敏感性为87.6%。从89.4%的HDM致敏的呼吸道过敏受试者和92.3%的2种主要变应原Der f 1和Der f 2的组合中检测到Der f 2单独的特异性IgE。然而，来自较少AD患者的血清，即72.4%和71.0%，分别识别Der f 1和Der f 2。2种主要过敏原的组合允许诊断AD患者的84.5%。在韩国HDM致敏的呼吸道和AD患者中，Der f 2是最常见的致敏过敏原，第1组和第2组主要过敏原的组合增加了诊断的敏感性。次要过敏原并不能显著提高诊断灵敏度。然而，需要进一步的研究来分析其他HDM过敏原致敏与HDM过敏的疾病实体之间的关系。
Measurement of IgE specific to purified house dust mite (HDM) allergens may improve allergy diagnosis. This study aimed to investigate the sensitization profiles of Korean HDM allergic subjects suffering from respiratory allergy and atopic dermatitis (AD) to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20. Recombinant HDM allergens were produced in Pichia pastoris (Der f 1) or Escherichia coli (5 allergens). IgE reactivity to the individual recombinant allergens and total extract of mite was assessed by ELISA. Der f 1 was recognized by 79.1%, Der f 2 by 79.1%, Der f 6 by 9.3%, Der f 8 by 6.2%, Der f 10 by 6.2%, and Der f 20 by 6.6% of the patients' sera tested, while the prevalence of IgE reactivity to total mite extract was 94.7%. Combination of Der f 1 and Der f 2 had a sensitivity of 87.6%. Specific IgE to Der f 2 alone was detected from 89.4% of HDM-sensitized respiratory allergy subjects and 92.3% to the combination of the 2 major allergens Der f 1 and Der f 2. However, sera from fewer patients with AD, namely 72.4% and 71.0%, recognized Der f 1 and Der f 2, respectively. The combination of 2 major allergens allowed diagnosis of 84.5% of the AD patients. No correlation between sensitization to specific allergens and HDM allergy entity was found. Der f 2 was the most frequently sensitized allergen among the HDM-sensitized respiratory and AD patients in Korea, and the combination of the group 1 and 2 major allergens increased the diagnostic sensitivity. Minor allergens did not significantly improve diagnostic sensitivity. However, further studies are needed to analyze the relationship between sensitization to other HDM allergens and the disease entity of the HDM allergy.