Enhancing peptaibols production in the biocontrol fungus Trichoderma longibrachiatum SMF2 by elimination of a putative glucose sensor
Enhancing peptaibols production in the biocontrol fungus Trichoderma longibrachiatum SMF2 by elimination of a putative glucose sensor
复制标题
通过消除假定的葡萄糖传感器来增强生物防治真菌长枝木霉 SMF2 中 peptaibols 的产生
DOI：
10.1002/bit.27138
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发表时间：
2019
影响因子：
3.8
通讯作者：
Zhang Yu-Zhong
中科院分区：
文献类型：
--
作者：
Zhou Yan-Rong;Song Xiao-Yan;Li Yue;Shi Jin-Chao;Shi Wei-Ling;Chen Xiu-Lan;Liu Wei-Feng;Liu Xiang-Mei;Zhang Wei-Xin;Zhang Yu-Zhong
木霉属是肽类抗生素的主要生产商虽然已经显示出辣椒素具有一系列的生物活性，但对其生产调节的分子理解在很大程度上是不清楚的，这阻碍了通过基因工程进行生产改进。在这里，我们证明了在杰出的生防真菌长枝木霉SMF 2中的葡萄糖传感器的直向同源物TlSTP 1参与调节肽酶的产生。Tlstp 1的缺失显著损害菌丝生长和分生孢子，但显著增加了Trichokonins A的5倍和Trichokonins B的2.6倍的肽产量。定量的真实的-时间聚合酶链反应分析表明，增加的肽酶产生发生在两个非核糖体肽合成酶编码基因tlx 1和tlx 2的转录水平。野生型和Tlstp 1突变株的转录组分析表明，Tlstp 1对一组参与许多代谢和细胞过程的基因发挥调节作用，包括其他几种次级代谢产物的合成。这些结果表明TlSTP 1在调节长臂嗜热厌氧菌SMF 2的营养生长和肽生产中的重要作用，并为通过基因工程构建肽高产菌株提供了见解。
Trichodermaspp. are main producers of peptide antibiotics known as peptaibols. While peptaibols have been shown to possess a range of biological activities, molecular understanding of the regulation of their production is largely unclear, which hampers the production improvement through genetic engineering. Here, we demonstrated that the orthologue of glucose sensors in the outstanding biocontrol fungusTrichoderma longibrachiatumSMF2,TlSTP1, participates in the regulation of peptaibols production. Deletion ofTlstp1markedly impaired hyphal growth and conidiation, but significantly increased peptaibols yield by 5‐fold for Trichokonins A and 2.6‐fold for Trichokonins B. Quantitative real‐time polymerase chain reaction analyses showed that the increased peptaibols production occurs at the transcriptional levels of the two nonribosomal peptide synthetase encoding genes,tlx1andtlx2. Transcriptome analyses of the wild type and theTlstp1mutant strains indicated thatTlSTP1 exerts a regulatory effect on a set of genes that are involved in a number of metabolic and cellular processes, including synthesis of several other secondary metabolites. These results suggest an important role ofTlSTP1 in the regulation of vegetative growth and peptaibols production inT. longibrachiatumSMF2 and provide insights into construction of peptaibol‐hyperproducing strains through genetic engineering.