De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments.
De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments.
复制标题
DOI：
10.1038/s41467-018-05922-x
复制
发表时间：
2018-08-24
影响因子：
16.6
通讯作者：
Warren MJ
中科院分区：
文献类型：
--
作者：
Lee MJ;Mantell J;Brown IR;Fletcher JM;Verkade P;Pickersgill RW;Woolfson DN;Frank S;Warren MJ
Bacterial microcompartments, BMCs, are proteinaceous organelles that encase a specific metabolic pathway within a semi-permeable protein shell. Short encapsulation peptides can direct cargo proteins to the lumen of the compartments. However, the fusion of such peptides to non-native proteins does not guarantee encapsulation and often causes aggregation. Here, we report an approach for targeting recombinant proteins to BMCs that utilizes specific de novo coiled-coil protein–protein interactions. Attachment of one coiled-coil module to PduA (a component of the BMC shell) allows targeting of a fluorescent protein fused to a cognate coiled-coil partner. This interaction takes place on the outer surface of the BMC. The redesign of PduA to generate an N-terminus on the luminal side of the BMC results in intact compartments to which proteins can still be targeted via the designed coiled-coil system. This study provides a strategy to display proteins on the surface or within the lumen of the BMCs. Bacterial microcompartments (BMCs) are protein-bound organelles encapsulating segments of metabolic pathways. Here the authors utilize specific de novo coiled-coil protein-protein interactions to display proteins on the outer or inner surface of BMCs.
登录
查看更多内容
影响因子：
8.4
作者：
Lee, Hanson;DeLoache, William C.;Dueber, John E.
通讯作者：
Dueber, John E.
影响因子：
14.8
作者：
Kelley LA;Mezulis S;Yates CM;Wass MN;Sternberg MJ
通讯作者：
Sternberg MJ
影响因子：
3.2
作者：
Havemann, GD;Sampson, EM;Bobik, TA
通讯作者：
Bobik, TA
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
影响因子：
4.8
作者：
Jakobson, Christopher M.;Kim, Edward Y.;Tullman-Ercek, Danielle
通讯作者：
Tullman-Ercek, Danielle