Genes encoding transcription factor IIIA and the RNA polymerase common subunit RPB6 are divergently transcribed in Saccharomyces cerevisiae.
Genes encoding transcription factor IIIA and the RNA polymerase common subunit RPB6 are divergently transcribed in Saccharomyces cerevisiae.
复制标题
编码转录因子 IIIA 和 RNA 聚合酶共同亚基 RPB6 的基因在酿酒酵母中以不同的方式转录。
DOI：
10.1073/pnas.89.9.3999
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发表时间：
1992
影响因子：
11.1
通讯作者：
Young,RA
中科院分区：
文献类型：
--
作者：
Woychik,NA;Young,RA
已发现编码酿酒酵母转录因子TFIIIA的基因邻近RPB 6，RPB 6是一种指定核RNA聚合酶共享的亚基的基因。对RPB 6基因上游DNA的分析揭示了一个开放的阅读框架，该框架预测了一种蛋白质，命名为PZF 1，具有9个C2 H2锌指。两栖类TFIIIA蛋白质的标志PZF 1蛋白中存在9个C2 H2锌指，表明PZF 1可能是TFIIIA同源物。我们发现，纯化的重组PZF 1特异性结合的5S rRNA基因的内部控制区（ICR）在S。啤酒。九个C2 H2锌指的存在下，特异性结合ICR DNA，和预测的分子量的PZF 1与纯化的酵母TFIIIA确定的相似性，一起表明PZF 1是TFIIIA。酵母和两栖类TFIIIA蛋白质共享的那些通常保守的C2 H2锌指以外的残基的数量有限，这些保守的残基可能提供线索，这些蛋白质的序列特异性。PZF 1基因被发现是单拷贝的，转录成1.5-脱氢酶mRNA，并且对于酵母细胞活力是必需的。有趣的是，酵母RPB 6和TFIIIA编码序列是不同的转录，并分开只有233个碱基对，提供了潜在的共调节表达的RNA聚合酶和核糖体的5S rRNA组分的组件。
The gene encoding Saccharomyces cerevisiae transcription factor TFIIIA has been found adjacent to RPB6, a gene that specifies a subunit shared by nuclear RNA polymerases. Analysis of DNA upstream of the RPB6 gene revealed an open reading frame that predicts a protein, designated PZF1, with nine C2H2 zinc fingers. The presence of nine C2H2 zinc fingers in PZF1 protein, a hallmark of amphibian TFIIIA proteins, suggested that PZF1 might be a TFIIIA homologue. We found that purified recombinant PZF1 specifically binds the internal control region (ICR) of the 5S rRNA gene in S. cerevisiae. The presence of nine C2H2 zinc fingers, the specific binding to ICR DNA, and the similarity of the predicted molecular mass of PZF1 with that determined for purified yeast TFIIIA, together indicate that PZF1 is TFIIIA. The yeast and amphibian TFIIIA proteins share only a limited number of residues outside of those normally conserved in C2H2 zinc fingers; these conserved residues may provide clues to the sequence specificity of these proteins. The PZF1 gene was found to be single copy, transcribed into a 1.5-kilobase mRNA, and essential for yeast cell viability. Interestingly, the yeast RPB6 and TFIIIA coding sequences are divergently transcribed and are separated by only 233 base pairs, providing the potential for coregulated expression of components of RNA polymerases and the 5S rRNA component of ribosomes.
兔皮质集合管顶膜钙激活钾通道的单通道记录。
DOI：
10.1073/pnas.81.13.4237
发表时间：
1984
影响因子：
11.1
作者：
Hunter,M;Lopes,AG;Boulpaep,EL;Giebisch,GH
通讯作者：
Giebisch,GH
局部麻醉剂暂时阻断通过单个乙酰胆碱受体通道的电流。
DOI：
--
发表时间：
1978
期刊：
Journal of Physiology
影响因子：
--
作者：
E. Neher;J. Steinbach
通讯作者：
J. Steinbach