MOLECULAR ANALYSIS OF THE PERIOD LOCUS IN DROSOPHILA-MELANOGASTER AND IDENTIFICATION OF A TRANSCRIPT INVOLVED IN BIOLOGICAL RHYTHMS
MOLECULAR ANALYSIS OF THE PERIOD LOCUS IN DROSOPHILA-MELANOGASTER AND IDENTIFICATION OF A TRANSCRIPT INVOLVED IN BIOLOGICAL RHYTHMS
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DOI：
10.1016/0092-8674(84)90265-4
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
ROSBASH, M
中科院分区：
文献类型：
--
作者：
REDDY, P;ZEHRING, WA;ROSBASH, M
分离并分析了黑腹隐翅虫周期(PER)基因的DNA序列。这个时钟基因的位置是通过染色体畸变在每个座位或非常接近的地方的分子图谱来界定的。至少有5个RNA从这个区域转录而来。其中一个转录本是0.9kb的转录本，与S对生物节律的控制密切相关。位于PER基因座的两个独立的心律失常突变极大地降低了该转录本的水平。0.9kb转录本的水平在光/暗周期中受到强烈的调节。来自先前报道的对PER‘’S功能的遗传和表型分析的证据表明，该区域可能是复杂的，并且来自PER区的几个基因产物，包括这个0.9kb转录本，可能参与受这个时钟基因影响的正常节律性的不同方面。
DNA sequences encompassing the period (per) locus of D. melanogaster were isolated and analyzed. The location of this clock gene was delimited by the molecular mapping of chromosome aberrations at or very near the per locus. At least 5 RNAs are transcribed from this region. One of these transcripts, a 0.9 kb [kilobase] species, is strongly implicated in per''s control of biological rhythms. Two independently isolated arrhythmic mutations at the per locus dramatically reduce the level of this transcript. The level of the 0.9 kb transcript is stronlgy modulated during a light/dark cycle. Evidence, from previously reported genetic and phenotypic analysis of per''s function, suggesting that this region may be complex and that several gene products from the per region, including this 0.9 kb transcript, may be involved in the different aspects of normal rhythmicity influenced by this clock gene is discussed.