STRUCTURE OF THE MEMBRANE-BOUND PROTEIN PHOTOSYNTHETIC REACTION CENTER FROM RHODOBACTER-SPHAEROIDES
STRUCTURE OF THE MEMBRANE-BOUND PROTEIN PHOTOSYNTHETIC REACTION CENTER FROM RHODOBACTER-SPHAEROIDES
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DOI：
10.1021/bi00236a005
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发表时间：
1991-06-04
期刊：
影响因子：
2.9
通讯作者：
SCHIFFER, M
中科院分区：
文献类型：
--
作者：
CHANG, CH;ELKABBANI, O;SCHIFFER, M
用分子置换法，以绿色红细菌（Rhodopyrusviridis）的光合反应中心（RC）为搜索结构，在3.1埃分辨率下测定了球形红细菌（Rhodobacter sphaeroides）的RC结构。 原子坐标用差分傅立叶方法和约束最小二乘细化技术细化到22%的当前R因子。 RC络合物的三级结构通过L和M链之间的疏水相互作用、颜料彼此之间以及颜料与L和M链之间的相互作用、来自L和M链的与Fe 2+配位的残基、L和M链与H链之间形成的盐桥以及可能通过螺旋末端之间的静电力来稳定。 在L和M链的N-末端的保守残基被鉴定为H链的识别位点。
The structure of the photosynthetic reaction center (RC) from Rhodobacter sphaeroides was determined at 3.1-angstrom resolution by the molecular replacement method, using the Rhodopseudomonas viridis RC as the search structure. Atomic coordinates were refined with the difference Fourier method and restrained least-squares refinement techniques to a current R factor of 22%. The tertiary structure of the RC complex is stabilized by hydrophobic interactions between the L and M chains, by interactions of the pigments with each other and with the L and M chains, by residues from the L and M chains that coordinate to the Fe2+, by salt bridges that are formed between the L and M chains and the H chain, and possibly by electrostatic forces between the ends of helices. The conserved residues at the N-termini of the L and M chains were identified as recognition sites for the H chain.