Extracellular signal-regulated kinase activation is required for up-regulation of vascular endothelial growth factor by serum starvation in human colon carcinoma cells.
Extracellular signal-regulated kinase activation is required for up-regulation of vascular endothelial growth factor by serum starvation in human colon carcinoma cells.
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发表时间：
1999-10
期刊：
影响因子：
11.2
通讯作者：
Young D. Jung;K. Nakano;Wenbiao Liu;G. Gallick;Lee M. Ellis
中科院分区：
文献类型：
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作者：
Young D. Jung;K. Nakano;Wenbiao Liu;G. Gallick;Lee M. Ellis
血管内皮生长因子（VEGF）是一种强有力的血管生成因子，对结肠癌新生血管的形成具有重要作用。在以前的研究中，血清饥饿导致人结肠癌细胞中VEGF的诱导。我们研究了有丝分裂原活化蛋白激酶可能参与血清饥饿诱导VEGF在HT 29人结肠癌细胞系。细胞外信号调节激酶（Erks）1和2在血清饥饿3-6 h后被激活。使用瞬时转染的VEGF启动子报告构建体，血清饥饿导致VEGF启动子活性的增加。Erk-1/2磷酸化抑制剂阻断了血清饥饿诱导的VEGF表达和启动子活性的增加。血清饥饿激活了几种丝裂原活化蛋白激酶，但Erk-1/2的激活是结肠癌细胞中VEGF mRNA上调的关键。
Vascular endothelial growth factor (VEGF) is a potent angiogenic factor important for colon cancer neovascularization. In previous studies, serum starvation led to induction of VEGF in human colon carcinoma cells. We investigated the possible participation of mitogen-activated protein kinases in serum starvation induction of VEGF in the HT29 human colon carcinoma cell line. The extracellular signal-regulated kinases (Erks) 1 and 2 were activated after 3-6 h of serum starvation. Using transient transfection of VEGF promoter-reporter constructs, serum starvation led to an increase in VEGF promoter activity. An inhibitor of phosphorylation of Erk-1/2 blocked the increase of VEGF expression and promoter activity induced by serum starvation. Serum starvation activates several mitogen-activated protein kinases, but activation of Erk-1/2 is critical for the up-regulation of VEGF mRNA in colon carcinoma cells.