Antigen-specific Immunoadsorption of Anti-acetylcholine Receptor Antibodies from Sera of Patients with Myastenia Gravis
Antigen-specific Immunoadsorption of Anti-acetylcholine Receptor Antibodies from Sera of Patients with Myastenia Gravis
复制标题
重症肌无力患者血清抗乙酰胆碱受体抗体的抗原特异性免疫吸附
DOI：
10.3109/10731191003634778
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发表时间：
2010-04-01
期刊：
影响因子：
--
通讯作者：
Li, Fangfang
中科院分区：
文献类型：
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作者：
Sun, Changyuan;Meng, Fanping;Li, Fangfang
背景资料：抗乙酰胆碱受体抗体（AChRAb）与神经肌肉接头处AChR α亚基的主要免疫原性区域（MIR）结合是重症肌无力（MG）的主要发病机制。研究方法：将人乙酰胆碱受体（AChR）MIR区的10个氨基酸合成肽段与纤维素微球偶联，制成抗原特异性免疫吸附剂（hMIR 10-CB）。结果如下：hMIR 10-CB可以去除MG血清中的AChRAb 40.3 +/-2.3%，而色氨酸非特异性吸附剂Trp-CB仅为22.4 +/- 1.5%，如在ELISA中测定的，并且还显示出对血细胞、血浆离子和血浆蛋白的良好血液相容性，如在兔全血灌注中检查的。结论：抗原特异性免疫吸附剂hMIR 10-CB可作为MG免疫吸附治疗的候选药物。
Background: The binding of anti-acetylcholine receptor antibodies (AChRAb) to the main immunogenic region (MIR) of AChR alpha-subunit in the neuromuscular junction is the major pathogenesis of myasthenia gravis (MG). Methods: A synthetic peptide of 10 amino acids corresponding to the MIR of human AChR was coupled with cellulose beads to make an antigen-specific immunoadsorbent (hMIR10-CB). Results: The hMIR10-CB could remove AChRAb in MG sera by 40.3 +/- 2.3%, compared to a tryptophan nonspecific adsorbent Trp-CB by only 22.4 +/- 1.5% as determined in ELISA, and also showed good blood compatibility for blood cells, plasma ions and plasma proteins as checked in whole blood perfusion in rabbits. Conclusions: The antigen-specific immunoadsorbent hMIR10-CB can serve as a potential candidate in the immunoadsorption treatment of MG.