[(3)H]Choline uptake and metabolism in nonsynaptic regions of a crustacean sensory nerve.
[(3)H]Choline uptake and metabolism in nonsynaptic regions of a crustacean sensory nerve.
复制标题
[(3)H]甲壳动物感觉神经非突触区域的胆碱摄取和代谢。
DOI：
10.1111/j.1471-4159.1981.tb00415.x
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发表时间：
1981
影响因子：
4.7
通讯作者：
Barker,DL
中科院分区：
文献类型：
--
作者：
Auerbach,A;Barker,DL
胃后神经 (PSN) 是一种甲壳动物感觉神经，包含约 60 个胆碱能神经元，这些神经元缺乏突触相互作用。动力学分析表明，PSN 通过低亲和力 (Km= 163 μM) 和高亲和力（Na+ 依赖性）(Km= 1 μM) 过程吸收 [3H] 胆碱。高亲和力系统的容量仅为低亲和力系统的1%左右。高亲和力系统与乙酰胆碱 (ACh) 合成并不紧密耦合，ACh 和磷酰胆碱似乎都是由细胞内胆碱池形成的，而胆碱池由两个摄取系统供给。包含神经元细胞体和不包含神经元细胞体的 PSN 区域之间的 [3H] 胆碱摄取率和 3H 代谢物积累率存在差异。这些差异可能源于每个神经区域中神经元与非神经元组织的相对比例的差异。
The posterior stomach nerve (PSN) is a crustacean sensory nerve containing about 60 cholinergic neurons, which are devoid of synaptic interactions. Kinetic analysis shows that the PSN takes up [3H]choline by both low‐affinity (Km= 163 μM) and high‐affinity (Na+‐dependent) (Km= 1 μM) processes. The capacity of the high‐affinity system is only about 1% that of the low‐affinity system. The high‐affinity system is not tightly coupled to acetylcholine (ACh) synthesis, and it appears that both ACh and phosphorylcholine are formed from an intracellular pool of choline, which is fed by both uptake systems. There are differences in the rates of [3H]choline uptake and3H metabolite accumulation between regions of the PSN that contain neuronal cell bodies and those that do not. These differences may arise from differences in the relative proportion of neuronal to nonneuronal tissue in each nerve region.