Induction of erythroid differentiation by cytoplast fusion in mouse erythroleukemia (Friend) cells.
Induction of erythroid differentiation by cytoplast fusion in mouse erythroleukemia (Friend) cells.
复制标题
通过小鼠红白血病（Friend）细胞的细胞质融合诱导红系分化。
DOI：
10.1016/s0014-4827(85)80051-3
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发表时间：
1985
影响因子：
3.7
通讯作者：
M. Oishi
中科院分区：
文献类型：
--
作者：
T. Watanabe;S. Nomura;M. Oishi
在小鼠Friend细胞中，由二甲基亚砜（DMSO）或六亚甲基双乙酰胺（HMBA）诱导的细胞内活性导致红系分化，其特征在于遗传标记的完整细胞与胞质之间的细胞融合。为此，设计了一种通过用寡霉素敏化非融合细胞来快速选择胞质杂种的方法。我们能够证明，来自DMSO-（或HMBA）处理的细胞的胞质体触发红系分化后，与紫外线照射的细胞融合。细胞质中的活性仅保持短暂，其诱导被生物活性佛波酯或放线菌酮抑制。然而，直接用DMSO（或HMBA）处理细胞质不能诱导这种活性。这些结果表明：（1）细胞内红系诱导活性位于胞质中，（2）它作为一个主导因子参与了红系的诱导分化，（3）它的产生需要新的蛋白质合成。的细胞内活动的诱导和它如何触发红系分化的机制进行了讨论。
An intracellular activity, which is induced by dimethyl sulfoxide (DMSO) or hexamethylenebisacetamide (HMBA) and leads to erythroid differentiation in mouse Friend cells, was characterized by cell fusion between genetically marked intact cells and cytoplasts. For this, a procedure for rapid selection of cybrids was devised by sensitizing non-fused cells with oligomycin. We were able to demonstrate that cytoplasts derived from DMSO- (or HMBA)-treated cells trigger erythroid differentiation upon fusion with UV-irradiated cells. The activity in the cytoplasts remained only transiently and its induction was inhibited by biologically active phorbol esters or cycloheximide. The activity, however, was not induced in cytoplasts by directly treating them with DMSO (or HMBA). These results indicate that (1) the intracellular erythroid-inducing activity is located in cytoplasts, (2) it acts intransand induces erythroid differentiation as a dominant factor and (3) its production requiresde novonuclear protein synthesis. The mechanisms of the induction of the intracellular activity and of how it triggers erythroid differentiation are discussed.