Growth and Biochemical Composition of Porphyridium purpureum SCS-02 under Different Nitrogen Concentrations
Growth and Biochemical Composition of Porphyridium purpureum SCS-02 under Different Nitrogen Concentrations
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不同氮浓度下紫球藻SCS-02的生长及生化组成
DOI：
10.3390/md17020124
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发表时间：
2019-02-01
期刊：
影响因子：
5.4
通讯作者：
Xiang, Wenzhou
中科院分区：
文献类型：
--
作者：
Li, Tao;Xu, Jin;Xiang, Wenzhou
紫球藻属微藻富含B-藻红蛋白（B-PE）、长链多不饱和脂肪酸（LC-PUFAs）和胞外多糖（EPS），具有巨大的商业化应用潜力。本研究的目的是调整培养氮浓度，以产生富含B-PE、LC-PUFAs和EPS的紫球藻生物量。紫球藻SCS-02在具有低氮供应（LN，3.5mM）、中等氮供应（MN，5.9mM）或高氮供应（HN，17.6mM）的ASW培养基中培养。HN显著促进了生物量、胞内蛋白质、β-PE和二十碳五烯酸的积累。LN能增加细胞内糖和花生四烯酸的含量，促进EPS的分泌。总脂含量几乎不受氮浓度的影响。基于这些结果，提出了一个半连续的两步过程，其中包括生产富含B-PE和LC-PUFAs的生物量与充足的氮，并诱导EPS分泌有限的氮和强光。
Microalgae of the genus Porphyridium show great potential for large-scale commercial cultivation, as they accumulate large quantities of B-phycoerythrin (B-PE), long chain polyunsaturated fatty acids (LC-PUFAs) and exopolysaccharide (EPS). The present study aimed to adjust culture nitrogen concentrations to produce Porphyridium biomass rich in B-PE, LC-PUFAs and EPS. Porphyridium purpureum SCS-02 was cultured in ASW culture medium with low nitrogen supply (LN, 3.5 mM), medium nitrogen supply (MN, 5.9 mM) or high nitrogen supply (HN, 17.6 mM). HN significantly enhanced the accumulation of biomass, intracellular protein, B-PE and eicosapentaenoic acid. LN increased the intracellular carbohydrate and arachidonic acid content, and promoted the secretion of EPS. The total lipids content was almost unaffected by nitrogen concentration. Based on these results, a semi-continuous two-step process was proposed, which included the production of biomass rich in B-PE and LC-PUFAs with sufficient nitrogen, and induced EPS excretion with limited nitrogen and strong light.