Histological assessments on the abnormalities of mouse epiphyseal chondrocytes with short term centrifugal loading.
Histological assessments on the abnormalities of mouse epiphyseal chondrocytes with short term centrifugal loading.
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DOI：
10.2220/biomedres.28.191
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发表时间：
2007-08
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影响因子：
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通讯作者：
P. H. L. de Freitas;T. Kojima;Sobhan Ubaidus;Minqi Li;Guangwei Shang;R. Takagi;T. Maeda;Kimimitsu Oda;H. Ozawa;N. Amizuka
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文献类型：
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作者：
P. H. L. de Freitas;T. Kojima;Sobhan Ubaidus;Minqi Li;Guangwei Shang;R. Takagi;T. Maeda;Kimimitsu Oda;H. Ozawa;N. Amizuka
我们检查了暴露于实验性超重力后软骨细胞的形态学变化。将17日龄小鼠胎儿胫骨骨骺软骨模拟超重力环境，3G离心16 h（实验组）或固定培养（对照组）。离心对骨骺软骨大小、软骨细胞增殖、X型胶原阳性肥厚带、甲状旁腺激素相关肽和成纤维细胞生长因子受体III mRNA表达无影响。然而，离心后的软骨细胞形态异常，空间排列异常，导致软骨细胞柱断裂。通过组织化学评估，肌动蛋白丝沿对照增殖软骨细胞细胞膜均匀分布，而受离心力作用的软骨细胞肌动蛋白丝层较厚。透射电镜观察显示，对照软骨细胞的细胞膜下有点状的电子致密物质，而实验软骨细胞的膜层较厚。在对照软骨柱内区域，纵向电子致密原纤维与正常软骨细胞的短细胞质过程相关，表明假设的细胞-基质相互作用。这些细胞外原纤维在离心样品中被破坏。总之，与细胞膜相关的肌动蛋白丝的改变，不规则的细胞形状和柱内细胞外原纤维的消失表明，在我们的软骨模型中，超重力扰乱了细胞与基质的相互作用。
We have examined the morphological changes in chondrocytes after exposure to experimental hypergravity. Tibial epiphyseal cartilages of 17-days-old mouse fetuses were exposed to centrifugation at 3G for 16 h mimicking hypergravitational environment (experimental group), or subjected to stationary cultures (control group). Centrifugation did not affect the sizes of epiphyseal cartilage, chondrocyte proliferation, type X collagen-positive hypertrophic zone, and the mRNA expressions of parathyroid hormone-related peptide and fibroblast growth factor receptor III. However, centrifuged chondrocytes showed abnormal morphology and aberrant spatial arrangements, resulting in disrupted chondrocytic columns. Through histochemical assessments, actin filaments were shown to distribute evenly along cell membranes of control proliferative chondrocytes, while chondrocytes subjected to centrifugal force developed a thicker layer of actin filaments. Transmission electron microscopic observations revealed spotty electron-dense materials underlying control chondrocytes' cell membranes, while experimental chondrocytes showed their thick layer. In the intracolumnar regions of the control cartilage, longitudinal electron-dense fibrils were associated with short cytoplasmic processes of normal chondrocytes, indicating assumed cell-tomatrix interactions. These extracellular fibrils were disrupted in the centrifuged samples. Summarizing, altered actin filaments associated with cell membranes, irregular cell shape and disappearance of intracolumnar extracellular fibrils suggest that hypergravity disturbs cell-to-matrix interactions in our cartilage model.