Utility of endogenous creatinine clearance as a measure of renal function in mice
Utility of endogenous creatinine clearance as a measure of renal function in mice
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DOI：
10.1111/j.1523-1755.2004.00600.x
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发表时间：
2004-05-01
影响因子：
19.6
通讯作者：
Sharma, K
中科院分区：
文献类型：
--
作者：
Dunn, SR;Qi, ZH;Sharma, K
背景。使用内源性血浆肌酐水平和肌酐清除率作为评估小鼠肾功能的工具已经受到严格审查，因为先前的研究报道，Jaffe碱性苦荞酸盐方法严重高估了小鼠的真实血浆肌酐。作为NIDDK糖尿病并发症动物模型联盟（AMDCC）的成员，我们评估了所有基于高效液相色谱（HPLC）的替代方法用于标准测定小鼠血浆肌酐和肌酐清除率的性能和可行性。我们的目的是开发一种简单的方法，为小体积(
Background. The use of endogenous plasma creatinine levels and creatinine clearance as a tool to evaluate renal function in mice has come under scrutiny as prior studies have reported that the Jaffe alkaline picrate method grossly overestimates true plasma creatinine in mice. As members of the NIDDK Animal Models of Diabetic Complications Consortium (AMDCC), we evaluated the performance and feasibility of ail alternative high-performance liquid chromatography (HPLC)-based method for standard determination of plasma creatinine and creatinine clearance in mice. Our purpose was to develop a simple method that provides a reliable, reproducible, and sensitive assay for small volumes (