Single-Cell Oxidative Stress Events Revealed by a Renewable SERS Nanotip
Single-Cell Oxidative Stress Events Revealed by a Renewable SERS Nanotip
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可再生 SERS 纳米尖揭示单细胞氧化应激事件
DOI：
10.1021/acssensors.1c00395
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发表时间：
2021-03-30
期刊：
影响因子：
8.9
通讯作者：
Xu, Shuping
中科院分区：
文献类型：
--
作者：
Chen, Jiamin;Wang, Jiaqi;Xu, Shuping
设计并制备了一种对活性氧（ROS）和NAD（+）/NADH（烟酰胺腺嘌呤二核苷酸的氧化/还原形式）敏感的纳米针尖，用于通过表面增强拉曼散射（Sers）光谱识别单个活细胞中的氧化还原事件。通过一步激光诱导银生长和沉积制备纳米尖端。氧化还原可逆的拉曼报告，4-巯基苯酚（4-MP），用于纳米尖装饰沿着与银沉积。4-MP可以通过Fe 3+离子转化为SERS无活性的4-巯基环己-2，5-二烯酮（4-MC），以完成多个氧化应激事件循环的信号归零。从4-MC到4-MP的Sers信号转换为还原过程提供了一个线索，该过程是依赖于NADH的。相反，通过将4-MP转化为4-MC，可以通过Sers研究药物（佛波醇12-肉豆蔻酸酯13-乙酸酯和H2 O2）刺激细胞的氧化应激事件和信号转导机制。该传感器易于制造，可回收利用。这种尖端型Sers纳米传感器可以扩展地用于追踪其他NAD（+）/NADH介导的呼吸链和糖酵解中的其他关键标记物，例如，乳酸、甘草酸、三磷酸腺苷和抗氧化剂。这将有助于在单细胞水平上研究氧化应激和线粒体代谢异常的疾病。
A nanotip sensitive to reactive oxygen species (ROS) and NAD(+)/NADH (oxidized/reduced forms of nicotinamide adenine dinucleotide) was designed and prepared to identify the redox events in a single living cell by surface-enhanced Raman scattering (SERS) spectroscopy. The nanotips were prepared by the one-step laser-induced Ag growth and deposition. A redox-reversible Raman reporter, 4-mercaptophenol (4-MP), was employed for the nanotip decoration along with the Ag deposition. 4-MP can be converted to SERS-inactive 4-mercaptocyclohexa-2,5-dienone (4-MC) by Fe3+ ions to complete signal rezeroing for multiple oxidative stress event loops. The SERS signal conversion from 4-MC to 4-MP provides a cue for the reduction process that is NADH-dependent. In contrast, by the conversion from 4-MP to 4-MC, the oxidative stress events and the signal transduction mechanism of cells stimulated by drugs (phorbol 12-myristate 13-acetate and H2O2) can be explored by SERS. This sensor is easy to fabricate and can be recycled. This tip-typed SERS nanosensor can be extendedly available for tracing other key markers in other NAD(+)/NADH-mediated respiratory chain and glycolysis, e.g., lactic acid, pyruvic acid, adenosine triphosphate, and antioxidants. It will be useful for investigating the diseases of abnormal oxidative stress and mitochondrial metabolism at the single-cell level.