Chemical Deprenylation of N6-Isopentenyladenosine (i6A) RNA
Chemical Deprenylation of N6-Isopentenyladenosine (i6A) RNA
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N(6)-异戊烯基腺苷 (i(6)A) RNA 的化学去异戊二烯化
DOI：
10.1002/anie.202003360
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发表时间：
2020-05-27
影响因子：
16.6
通讯作者：
Cheng, Liang
中科院分区：
文献类型：
--
作者：
Cheng, Hou-Ping;Yang, Xiao-Hui;Cheng, Liang
N-6-异戊烯基腺苷（i（6）A）是在细胞分裂素中发现的RNA修饰，其调节植物生长/分化，并且是tRNA的子集，其中它提高翻译的效率和准确性。这种修饰的安装和移除是由异戊烯基转移酶和细胞分裂素氧化酶介导的，并且尚未开发出选择性地使i（6）A去异戊二烯化的化学方法。我们表明，一组选定的氧代铵阳离子的功能作为人工deprenylases，以促进高选择性deprenylation的i（6）A核苷，寡核苷酸和活细胞。重要的是，其他表观遗传修饰，氨基酸残基和天然产物不受影响。此外，一个显着的表型差异，在拟南芥的芽和根的发育观察与孵育的阳离子。这些结果确定这些有机小分子作为i（6）A的直接化学调节剂/人工脱异戊二烯酶。
N-6-isopentenyladenosine (i(6)A) is an RNA modification found in cytokinins, which regulate plant growth/differentiation, and a subset of tRNAs, where it improves the efficiency and accuracy of translation. The installation and removal of this modification is mediated by prenyltransferases and cytokinin oxidases, and a chemical approach to selective deprenylation of i(6)A has not been developed. We show that a selected group of oxoammonium cations function as artificial deprenylases to promote highly selective deprenylation of i(6)A in nucleosides, oligonucleotides, and live cells. Importantly, other epigenetic modifications, amino acid residues, and natural products were not affected. Moreover, a significant phenotype difference in the Arabidopsis thaliana shoot and root development was observed with incubation of the cation. These results establish these small organic molecules as direct chemical regulators/artificial deprenylases of i(6)A.