BUB-1 targets PP2A:B56 to regulate chromosome congression during meiosis I in C. elegans oocytes
BUB-1 targets PP2A:B56 to regulate chromosome congression during meiosis I in C. elegans oocytes
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BUB-1 靶向 PP2A:B56 调节线虫卵母细胞减数分裂 I 期间的染色体会聚
DOI：
10.1101/2020.06.12.148254
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Borja L
中科院分区：
文献类型：
--
作者：
Borja L
蛋白磷酸酶2A （PP2A）是由支架(a)、催化(C)和调节(B)亚基组成的异源三聚体。具有B56亚基的PP2A复合物被Shugoshin和BUBR1靶向，以保护酵母和小鼠减数分裂中的着丝粒内聚和稳定着丝点-微管附着。在秀丽隐杆线虫中，最接近的BUBR1同源物缺乏b56相互作用结构域，并且Shugoshin不需要减数分裂分离。因此，PP2A inC.在雌性减数分裂中的作用尚不清楚。我们报道了PP2A在减数分裂纺锤体组装和染色体动力学过程中是必不可少的。elegansfemale减数分裂。bbu1是B56亚基在前期i的主要染色体靶向因子。bbu1通过新发现的LxxIxE基序以磷酸化依赖的方式将PP2A:B56招募到染色体上，这种招募对于正确的染色体聚集很重要。我们的研究结果强调了B56募集的新机制，这对于在减数分裂I期间募集参与染色体聚集的PP2A至关重要。
Protein Phosphatase 2A (PP2A) is a heterotrimer composed of scaffolding (A), catalytic (C), and regulatory (B) subunits. PP2A complexes with B56 subunits are targeted by Shugoshin and BUBR1 to protect centromeric cohesion and stabilise kinetochore–microtubule attachments in yeast and mouse meiosis. InCaenorhabditis elegans, the closest BUBR1 orthologue lacks the B56-interaction domain and Shugoshin is not required for meiotic segregation. Therefore, the role of PP2A inC. elegansfemale meiosis is unknown. We report that PP2A is essential for meiotic spindle assembly and chromosome dynamics duringC. elegansfemale meiosis. BUB-1 is the main chromosome-targeting factor for B56 subunits during prometaphase I. BUB-1 recruits PP2A:B56 to the chromosomes via a newly identified LxxIxE motif in a phosphorylation-dependent manner, and this recruitment is important for proper chromosome congression. Our results highlight a novel mechanism for B56 recruitment, essential for recruiting a pool of PP2A involved in chromosome congression during meiosis I.
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